Histological Specimen Treatment Apparatus and Method
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Abstract
Method and apparatus for processing tissue specimens against decomposition, putrefaction and autolysis which use a simple three step procedure in a single vessel or container. First, the specimens are saturated with a solvent mixture of a ketone and a hydrocarbon, e.g., an Acetone/Hexane or an Acetone/Xylene mixture, to dissolve lipids and other cellular solutes. The container is then flooded with melted Paraffin. In a last step, the solvent mixture is vaporized and evacuated from the container, allowing the melted Paraffin to replace the vaporized solvent and impregnate the specimens. Raw, i.e., non-processed and non-burred specimens up to 5 mm thick can be processed in about 60 minutes. A solvent regenerator distills the evacuated solvent; and converts vent waste gases into carbon dioxide and water through a thermocatalytic oxidizer.
3 Citations
43 Claims
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1-31. -31. (canceled)
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32. An apparatus for processing tissue specimens against decomposition, putrefaction and autolysis which comprises:
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a hermetically sealable reaction container; means for controlling the temperature and pressure inside said container; a source of dissolving compound connectable to said container; a source of melted Paraffin connectable to said container; and means for evacuating gases from said container; wherein said dissolving compound comprises a mixture of a ketone and at least one low molecular weight hydrocarbon. - View Dependent Claims (33, 34, 35, 36, 37, 38, 39, 40, 41, 42)
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43-50. -50. (canceled)
Specification