Biosensor Comprising Electrode for Measuring Hematocrit Value

• US 20160290987A1
• Filed: 04/05/2016
• Published: 10/06/2016
• Est. Priority Date: 04/06/2015
• Status: Active Grant

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Saeda, Megumi, Kaneda, Hisashi
• Granted Patent: US 9,952,197 B2
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 27/028 Circuits therefor measuring impedance per se G01R27/02
• CPC Class Codes: G01N 27/3274 Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration coding of calibration information G01N33/48771
• CPC Class Codes: G01N 33/48707 by electrical means G01N33/49, G01N33/493 take precedence

Abstract

Provided is a method for measuring the concentration of a substance in a blood sample, comprising: supplying the blood sample to a biosensor comprising a hematocrit electrode for measuring a hematocrit value on the surface of which electrode an electrically conductive polymer is covalently immobilized; and calculating the concentration of the substance from the measured value of a first current resulting from application of a first voltage; and correcting the concentration of the substance with the value of a second current resulting from application of a second voltage or the hematocrit value calculated from the value of the second current.

Claims

1. A biosensor comprising a hematocrit electrode configured to measure a hematocrit value in a blood sample, wherein an electrically conductive polymer is covalently immobilized on a surface of the hematocrit electrode.

2. The biosensor according to claim 1, wherein the electrically conductive polymer is a water-soluble electrically conductive polymer.

3. The biosensor according to claim 1, wherein the electrically conductive polymer is polyaniline.

4. The biosensor according to claim 1, wherein the electrically conductive polymer is immobilized by a crosslinking agent.

5. The biosensor according to claim 4, wherein the crosslinking agent contains at least one functional group selected from the group consisting of an aldehyde group, a maleimide group, a carbodiimide group, an oxazoline group, an epoxy group and combinations thereof.

6. The biosensor according to claim 1, further comprising a working electrode, a counter electrode and a reference electrode.

7. The biosensor according to claim 6, wherein the working electrode is an enzyme electrode.

8. The biosensor according to claim 7, wherein the enzyme electrode contains a detection layer comprising an enzyme, an electrically conductive polymer and a crosslinking agent.

9. The biosensor according to claim 8, wherein the enzyme is an oxidoreductase.

10. The biosensor according to claim 8, wherein the enzyme is saccharide oxidoreductase.

11. The biosensor according to claim 8, wherein the detection layer further comprises electrically conductive particles.

12. A method for measuring the concentration of a substance in a blood sample, comprising:

supplying the blood sample to the biosensor according to claim 6;

measuring the value of a first current which flows when a first voltage of 0 to 400 mV is applied to the working electrode, and calculating the concentration of the substance from the measured value of the first current;

measuring the value of a second current which flows when a second voltage of 400 to 800 mV is applied to the hematocrit electrode; and

correcting the concentration of the substance with the value of the second current or the hematocrit value calculated from the value of the second current.

13. The method according to claim 12, wherein the substance is glucose.

14. The method according to claim 12, wherein the first voltage is 100 to 300 mV and the second voltage is 550 to 650 mV.

15. A measuring apparatus comprising:

the biosensor according to claim 6;

a control section configured to control the application of voltage to the working electrode and the hematocrit electrode of the biosensor;

a detection section configured to measure the value of a first current which flows when a voltage is applied to the working electrode, and the value of a second current which flows when a voltage is applied to the hematocrit electrode;

an arithmetic section configured to calculate the concentration of a substance from the value of the first current, and to correct the concentration of the substance with the value of the second current or the hematocrit value calculated from the value of the second current; and

an output section configured to output the corrected concentration of the substance to be measured.

16. A method for measuring a hematocrit value of a blood sample, comprising:

supplying a blood sample to the biosensor according to claim 1; and

measuring the value of a current which flows when a voltage of 400 to 800 mV is applied to the hematocrit electrode.

17. A measuring apparatus comprising:

the biosensor according to claim 1;

a control section configured to control the application of voltage to the hematocrit electrode of the biosensor;

a detection section configured to measure the value of a current which flows when a voltage is applied to the hematocrit electrode;

an arithmetic section configured to calculate the hematocrit value from the measured value of the current; and

an output section configured to output the calculated hematocrit value.