Allelic Exclusion
2 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to a method for the generation of VH heavy chain-only antibodies in a transgenic non-human mammal. In particular, the present invention relates to a method for the production of a VH heavy chain-only antibody in a transgenic non-human mammal comprising the step of expressing more than one heterologous VH heavy chain locus in that mammal.
6 Citations
52 Claims
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1-34. -34. (canceled)
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35. A method for increasing the diversity of VH heavy chain-only antibodies in a transgenic non-human mammal comprising the steps of
a) providing a transgenic non-human mammal whose genome comprises more than one heterologous VH heavy chain locus, wherein each VH heavy chain locus is different and comprises one or more V gene segments, one or more D gene segments, one or more J gene segments and a gene segment encoding a heavy chain constant region which, when expressed, does not include a functional CH1 domain and a) expressing a VH heavy chain-only antibody from at least one of said loci in a B-cell specific manner, wherein said heterologous VH heavy chain loci are present on the same or different chromosomes, and the expression of a heterologous VH locus is determined by allelic exclusion.
Specification