MULTIPARTITE SIGNALING PROTEINS AND USES THEREOF
First Claim
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1. A non-natural cell, comprising:
- (a) a first nucleic acid molecule encoding a first fusion protein comprising a first multimerization domain, a hydrophobic domain, and an actuator domain, wherein the first multimerization domain localizes extracellularly when the first fusion protein is expressed; and
(b) a second nucleic acid molecule encoding a second fusion protein comprising a secretion signal, a binding domain and a second multimerization domain, wherein the second fusion protein localizes extracellularly when expressed;
wherein a bridging factor promotes the formation of a polypeptide complex on the non-natural cell surface with the bridging factor associated with and disposed between the multimerization domains of the first and second fusion proteins.
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Abstract
The present disclosure relates to compositions and methods for using cells having chemically-induced fusion protein complexes to spatially and temporally control immune cell signal initiation and downstream responses for treating disease.
6 Citations
96 Claims
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1. A non-natural cell, comprising:
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(a) a first nucleic acid molecule encoding a first fusion protein comprising a first multimerization domain, a hydrophobic domain, and an actuator domain, wherein the first multimerization domain localizes extracellularly when the first fusion protein is expressed; and (b) a second nucleic acid molecule encoding a second fusion protein comprising a secretion signal, a binding domain and a second multimerization domain, wherein the second fusion protein localizes extracellularly when expressed; wherein a bridging factor promotes the formation of a polypeptide complex on the non-natural cell surface with the bridging factor associated with and disposed between the multimerization domains of the first and second fusion proteins. - View Dependent Claims (2, 3, 4, 5, 6, 7, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 37, 38, 39, 40, 43, 44)
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8-15. -15. (canceled)
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35-36. -36. (canceled)
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41-42. -42. (canceled)
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45-94. -94. (canceled)
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95. A fusion polypeptide heterocomplex, comprising:
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(a) a first fusion protein comprising a first multimerization domain, a hydrophobic domain, and an actuator domain; (b) a second fusion protein comprising a secretion signal, an extracellular binding domain and second multimerization domain; and (c) a bridging factor; wherein the first fusion protein, second fusion protein, and bridging factor associate to form a polypeptide heterocomplex with the bridging factor associated with and disposed between the multimerization domains of the first and second fusion proteins.
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96-158. -158. (canceled)
Specification