METHODS OF MAKING VANILLIN VIA THE MICROBIAL FERMENTATION OF FERULIC ACID FROM EUGENOL USING A PLANT DEHYDROGENASE
First Claim
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1. A bioconversion method of making ferulic acid comprising the following steps:
- expressing a VaoA gene in a mixture;
expressing a MtSAD1 gene in the mixture;
feeding eugenol to the mixture; and
collecting ferulic acid.
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Abstract
A bioconversion method of making vanillin including expressing VaoA gene in a mixture, expressing MtSAD1 gene in the mixture, feeding eugenol to the mixture, and converting ferulic acid to vanillin by incubating with a microbial Amycolalopsis sp. strain (Zhp06) and/or a recombinant E. coli strain.
3 Citations
55 Claims
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1. A bioconversion method of making ferulic acid comprising the following steps:
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expressing a VaoA gene in a mixture; expressing a MtSAD1 gene in the mixture; feeding eugenol to the mixture; and collecting ferulic acid. - View Dependent Claims (2, 6, 10, 14, 33, 34, 35, 40, 45)
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3-5. -5. (canceled)
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11-13. -13. (canceled)
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17. (canceled)
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18. A bioconversion method of making vanillin comprising:
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expressing a VaoA gene in a mixture; expressing a MtSAD1 gene in the mixture; feeding eugenol to the mixture; and converting ferulic acid to vanillin. - View Dependent Claims (19, 23, 27, 28, 32, 46)
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20-22. -22. (canceled)
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24-26. -26. (canceled)
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29-30. -30. (canceled)
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36-39. -39. (canceled)
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41-44. -44. (canceled)
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47-50. -50. (canceled)
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51. A method of producing ferulic acid using a recombinant E. coli strain comprising:
- (i) cultivating the recombinant E. coli strain in a medium;
(ii) adding eugenol to the culture of (i) to begin the biotransformation of eugenol;
(iii) allowing the biotransformation to proceed; and
(iv) extracting the ferulic acid from the culture, wherein the recombinant E. coli strain comprises a plasmid that expresses MtSAD1. - View Dependent Claims (54)
- (i) cultivating the recombinant E. coli strain in a medium;
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52-53. -53. (canceled)
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55-80. -80. (canceled)
Specification