MEASURING CELL, DETECTOR, AND ANALYSIS DEVICE

• US 20160319232A1
• Filed: 04/27/2016
• Published: 11/03/2016
• Est. Priority Date: 04/30/2015
• Status: Abandoned Application

• Current Assignee: Kabushiki Kaisha Toshiba (Toshiba Corporation)
• Original Assignee: Kabushiki Kaisha Toshiba (Toshiba Corporation)
• Inventors: NORITOMI, Yasuko, YAMADA, Ko, FUKE, Hiroyuki, KUBOKI, Takashi
• Application Number:
  US15/139,832
• Publication Number:
  US 20160319232A1
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: C12M 21/18 Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
• CPC Class Codes: G01N 27/3277 being a redox reaction, e.g. detection by cyclic voltammetry voltammetry per se G01N27/42, G01N27/48
• CPC Class Codes: G01N 27/49 Systems involving the determination of the current at a single specific value, or small range of values, of applied voltage for producing selective measurement of one or more particular ionic species

Abstract

According to one embodiment, a measuring cell includes a main cell member, and a mixture supported by or held in the main cell member. The mixture includes a nonaqueous solvent-including medium and one or more enzyme bodies. The one or more enzyme bodies are selected from the group including an enzyme, a first composite including an enzyme and a molecular aggregate that includes a dispersant, a microcapsule including an enzyme-including core and a shell covering the core, a cell including an enzyme, a microorganism including an enzyme, and a second composite including an enzyme and a support immobilizing the enzyme.

Claims

1. A measuring cell comprising:

a main cell member; and

a mixture supported by or held in the main cell member, and including a nonaqueous solvent-including medium and one or more enzyme body, the one or more enzyme body being selected from the group consisting of an enzyme, a first composite including an enzyme and a molecular aggregate that includes a dispersant, a microcapsule including an enzyme-including core and a shell covering the core, a cell including an enzyme, a microorganism including an enzyme, and a second composite including an enzyme and a support immobilizing the enzyme.

2. The cell according to claim 1, wherein at least a part of the one or more enzyme body is hygroscopic or includes water in contact with the enzyme, or the enzyme included in at least a part of the one or more enzyme body catalyzes a reaction that generates water, or catalyzes a reaction that generates a compound which is decomposed into water and another compound by a redox reaction.

3. The cell according to claim 1, wherein the nonaqueous solvent includes an ionic liquid.

4. The cell according to claim 1, wherein the mixture further includes a substrate, and the substrate is a reactant of a reaction catalyzed by the enzyme included in at least a part of the one or more enzyme body.

5. The cell according to claim 1, wherein the mixture is a gel.

6. The cell according to claim 1, wherein the one or more enzyme body includes the first composite, and the molecular aggregate includes one or more of a reversed micelle, a reverse wormlike micelle, liposome, vesicle, a microemulsion, a larger emulsion, a bicontinuous microemulsion, a monodispersed single emulsion, a double emulsion, and a multilayered emulsion.

7. The cell according to claim 1, wherein the one or more enzyme body includes the microcapsule, the shell includes a gel or a polymeric material, and the core includes a molecular aggregate that includes a dispersant, a cell, or a microorganism.

8. The cell according to claim 1, wherein the one or more enzyme body includes the microcapsule, and the shell includes a porous hydrophilic membrane.

9. The cell according to claim 1, wherein the one or more enzyme body includes the second composite, and the support includes one or more material selected from the group consisting of a water-absorbing material, a hydrogel, a silica gel, a polymer gel, a molecular gel, a nanofiber gel, an inorganic material having a nanoporous structure, a cyclodextrin/polymer, a nanogel, a nanofiber, a porous spherical silica particle having a mesopore, and a polymeric material.

10. The cell according to claim 1, wherein the enzyme included in at least a part of the one or more enzyme body includes one or more enzyme selected from the group consisting of a hydrolytic enzyme, a redox enzyme, synthase, transferase, an elimination enzyme, a modified enzyme, a protein crosslinking enzyme, a mutation enzyme, an artificial enzyme, a crosslinking enzyme, an antibody enzyme, lyase, ligase, and a crystallized enzyme.

11. A detector comprising:

the measuring cell of claim 1; and

a measuring unit configured to measure an electrical property or an electrochemical property of the mixture

wherein the measuring cell further comprises one or more electrodes disposed in contact with the mixture.

12. An analysis device comprising:

the detector of claim 11; and

a sampling unit including at least one of a vaporizer configured to vaporize a measurement target substance included in a sample to be measured by laser irradiation, UV irradiation, gas spraying, ultrasonic irradiation, heating, and voltage application, and an ionization source configured to ionize the measurement target substance.