Pathogenesis Quantification Systems and Treatment Methods for Citrus Greening Blight
First Claim
1. A method for detecting pathogenesis in a biological sample, comprising:
- a) quantifying the amount of a nucleic acid in the sample specific for a first organism that is a pathogen associated with a second organism that in tandem the first and second organisms affect a host organism to determine a pathogen quantitative measurement,b) quantifying the amount of a nucleic acid in the sample specific for the host organism response to pathogen to determine a multiplex quantitation for targets associated with the pathogen of a disease; and
c) calculating the ratio of the amount of dual pathogen targets relative to the amount of host quantitative measures, wherein said ratio provides a pathogen index for detecting pathogenesis.
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Abstract
The invention relates to a novel pathogenesis model, method, or kit for detecting pathogenesis in a subject. In particular, the invention provides a pathogen index derived from a ratio of the amount of dual pathogen targets relative to the amount of host quantitative measures. The pathogen index is used in diagnosis, prognosis, and/or treatment strategy of any disease, including citrus greening diseases (HLB). Research tools and methods for screening drugs for treating or preventing citrus greening diseases, as well as treatment or prevention methods for citrus greening diseases are also provided.
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Citations
28 Claims
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1. A method for detecting pathogenesis in a biological sample, comprising:
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a) quantifying the amount of a nucleic acid in the sample specific for a first organism that is a pathogen associated with a second organism that in tandem the first and second organisms affect a host organism to determine a pathogen quantitative measurement, b) quantifying the amount of a nucleic acid in the sample specific for the host organism response to pathogen to determine a multiplex quantitation for targets associated with the pathogen of a disease; and c) calculating the ratio of the amount of dual pathogen targets relative to the amount of host quantitative measures, wherein said ratio provides a pathogen index for detecting pathogenesis. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A kit for detecting pathogenesis in a sample, comprising:
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a) at least a first set of oligonucleotides being at least partially complementary to a nucleic acid of a first pathogen organism that is pathogenically associated with a second organism that in tandem these two organisms affects a host organism to determine a pathogen quantitative measurement, b) at least a second set of oligonucleotides being at least partially complementary to a nucleic acid of the host organism response to pathogen of a disease, c) reagents for nucleic acid amplification reactions with the first and second sets of oligonucleotides; d) instructions for conducting the nucleic acid amplification reactions and detecting one or more amplicons thereof; and e) instructions for calculating a ratio of the amplicons or the amount of dual pathogen targets relative to the amount of host quantitative measures, wherein said ratio provides a pathogen index for detecting, prognosis, drug screening, and treatment strategy or efficacy for the pathogenesis. - View Dependent Claims (17)
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18. A method for screening a candidate therapeutic agent for inhibition or prevention of citrus greening blight (HLB), comprising providing a culture of Candidatus Liberibacter with a bacteriophage having a repressed phage lytic cycle;
- combining the culture with a candidate therapeutic agent candidate; and
detecting inhibition of growth of the Candidatus Liberibacter culture indicating that the candidate therapeutic agent inhibits or prevents citrus greening blight (HLB). - View Dependent Claims (19, 20)
- combining the culture with a candidate therapeutic agent candidate; and
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21. A method for treating bacteriophage infection in bacteria, plant or animal individual comprising administering to the individual in need a composition comprising an effective amount of an agent selected from the group consisting of nucleoside, non-nucleoside, nucleotide, non-nucleotide, ribonucleoside, and a ribonucleoside analog that selectively inhibits viral replication.
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22. A method for treating viral infection in a plant comprising administering to the plant in need a composition comprising an effective amount of an agent selected from the group consisting of nucleoside, non-nucleoside, nucleotide, non-nucleotide, ribonucleoside, and a ribonucleoside analog that selectively inhibits viral replication.
- 23. A method for treating citrus greening blight comprising administering to a citrus tree in need a composition comprising an effective amount of an agent selected from the group consisting of nucleoside, non-nucleoside, nucleotide, non-nucleotide, ribonucleoside, and a ribonucleoside analog that selectively inhibits viral replication.
- 26. A method for preventing citrus greening blight comprising administering to a citrus tree in need a composition comprising an effective amount of an agent selected from the group consisting of nucleoside, non-nucleoside, nucleotide, non-nucleotide, ribonucleoside, and a ribonucleoside analog that selectively inhibits viral replication.
Specification