METHOD AND KIT FOR TARGET MOLECULE CHARACTERIZATION

• US 20160326582A1
• Filed: 05/04/2016
• Published: 11/10/2016
• Est. Priority Date: 05/04/2015
• Status: Abandoned Application

• Current Assignee: Fry Laboratories, LLC
• Original Assignee: Fry Laboratories, LLC
• Inventors: Ellis, Jeremy
• Application Number:
  US15/146,794
• Publication Number:
  US 20160326582A1
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: C12Q 2525/155 incorporating/generating a new priming site
• CPC Class Codes: C12Q 2531/113 PCR
• CPC Class Codes: C12Q 2531/125 Rolling circle

Abstract

Methods and kits for characterizing molecules, such as nucleic acids, large oligonucleotides, or portions thereof and/or to methods and kits for preparing samples for a characterization process are disclosed. Exemplary methods and kits can be used to form addressable portions, such that the addressable portions can be identified, sequenced, and reassembled to reconstruct an exemplary or characteristic molecule of a target molecule.

Claims

1. A method of characterizing a target molecule, the method comprising:

using one or more identifying molecules attached to the target molecule, forming two or more addressable divided sections,

wherein the one or more identifying molecules include a random or unique identifying portion, and

wherein the two or more addressable divided sections can be used to characterize the target molecule.

2. The method of claim 1, wherein the two or more addressable divided sections comprise a first section and a second section.

3. The method of claim 1, further comprising a step of determining a representative first section.

4. The method of claim 3, wherein the representative first section is determined based on a most-common sequence for the first section.

5. The method of claim 3, wherein the representative first section is determined based on a determination of a most-common base at one or more sites of a plurality of sequences of one or more first sections.

6. The method of claim 1, further comprising a step of determining a representative second section.

7. The method of claim 6, wherein the representative second section is determined based on a most-common sequence for the second section.

8. The method of claim 6, wherein the representative second section is determined based on a determination of a most-common base at one or more sites of a plurality of sequences of one or more second sections.

9. A method of characterizing a target molecule, the method comprising the steps of:

attaching a first identifying molecule to a first end of the target molecule;

attaching a second identifying molecule to a second end of the target molecule;

forming a plurality of continuous molecules; and

dividing a first portion of the continuous molecules into addressable first sections and a second portion of the continuous molecules into addressable second sections.

10. The method of claim 9, further comprising the steps of sequencing the first section to obtain a first section sequence and sequencing the second section to obtain a second section sequence.

11. The method of claim 10, further comprising a step of determining a sequence of the target molecule using the first section sequence and the second section sequence.

12. The method of claim 10, further comprising a step of, using a computer, determining a sequence of the target molecule using the first section sequence and the second section sequence.

13. The method of claim 9, wherein the first identifying molecule comprises a first primer.

14. The method of claim 13, wherein the first primer is a specific primer or a random primer.

15. The method of claim 9, wherein the first identifying molecule comprises a first tag.

16. The method of claim 15, wherein the first tag comprises a random molecule.

17. The method of claim 15, wherein each of base sites or base pairs of the first tag is random.

18. The method of claim 11, wherein the step of determining comprises identifying one or more of at least a portion of the first identifying molecule and identifying at least a portion of the second identifying molecule.

19. The method of claim 11, wherein the step of determining comprises identifying one or more of a first tag of the first identifying molecule and identifying a second tag of the second identifying molecule.

20. A method of characterizing a target molecule, the method comprising the steps of:

using a first amplification process, attaching a first identifying molecule to a first end of the target molecule and attaching a second identifying molecule to a second end of the target molecule;

forming a plurality of continuous molecules; and

dividing a portion of the plurality of continuous molecules into a plurality of first sections and another portion of the plurality of continuous molecules into a plurality of second sections.