Systems And Methods for Three Dimensional Imaging

• US 20160327779A1
• Filed: 07/15/2016
• Published: 11/10/2016
• Est. Priority Date: 01/17/2014
• Status: Active Grant

• Current Assignee: Trustees Of Columbia University In The City Of New York (Columbia University)
• Original Assignee: Trustees Of Columbia University In The City Of New York (Columbia University)
• Inventors: Hillman, Elizabeth
• Granted Patent: US 10,061,111 B2
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: G02B 21/0032 Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers G02B21/0036 - G02B21/008; means for illumination of specimens in general G02B21/06
• CPC Class Codes: G02B 21/0052 Optical details of the image generation
• CPC Class Codes: G02B 21/361 Optical details, e.g. image relay to the camera or image sensor G02B21/364 takes precedence; illumination details G02B21/06 and subgroups
• CPC Class Codes: G02B 21/367 providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
• CPC Class Codes: G02B 23/04 for the purpose of beam splitting or combining, e.g. fitted with eyepieces for more than one observer G02B23/10 takes precedence

Abstract

Methods, devices and systems for up to three-dimensional scanning of target regions at high magnification are disclosed.

Claims

1. An optical imaging apparatus comprising:

a beam splitter;

a first light scanning element;

a second light scanning element;

an objective;

an illumination source that sends illumination light into the objective via a first optical path, wherein the first optical path includes the beam splitter and the first light scanning element,

wherein the beam splitter and the first light scanning element redirect the illumination light towards a peripheral region of the objective such that the illumination light passes through the objective and forms an oblique imaging plane in a tissue, and wherein the position of the oblique imaging plane within the tissue varies depending on an orientation of the first light scanning element,

wherein the objective accepts return light from the oblique imaging plane and passes the return light onto a second optical path that includes the beam splitter and the second light scanning element, and wherein the beam splitter and the second light scanning element route the return light along the second optical path so as to form a stationary tilted intermediate image plane; and

a light detector that captures an image of the stationary tilted intermediate image plane.

2. The apparatus of claim 1, wherein a single reflecting surface serves as both the first light scanning element and the second light scanning element.

3. The apparatus of claim 2, wherein the illumination light arrives at the beam splitter before arriving at the single reflecting surface, and wherein the return light arrives at the single reflecting surface before arriving at the beam splitter.

4. The apparatus of claim 3, further comprising a telescope disposed in both the first optical path and the second optical path, wherein the illumination light arrives at the single reflecting surface before arriving at the telescope, wherein the illumination light arrives at the telescope before arriving at the objective, wherein the return light arrives at the objective before arriving at the telescope, and wherein the return light arrives at the telescope before arriving at the single reflecting surface.

5. The apparatus of claim 1, wherein the first light scanning element and the second light scanning element use different reflecting surfaces that move in synchronization with each other.

6. The apparatus of claim 5, wherein the illumination light arrives at the first light scanning element before arriving at the beam splitter, and wherein the return light arrives at the beam splitter before arriving at the second light scanning element.

7. The apparatus of claim 6, further comprising an illumination telescope disposed in the first optical path and a return-light telescope disposed in the second optical path, wherein the illumination light arrives at the first light scanning element before arriving at the illumination telescope, wherein the illumination light arrives at the illumination telescope before arriving at the objective, wherein the return light arrives at the objective before arriving at the return-light telescope, and wherein the return light arrives at the return-light telescope before arriving at the second light scanning element.

8. The apparatus of claim 7, where in the first light scanning element and the second light scanning element are mechanically independent from each other.

9. The apparatus of claim 5, where in the first light scanning element and the second light scanning element are mechanically independent from each other.

10. The apparatus of claim 1, wherein the light detector is positioned directly at the stationary tilted intermediate image plane.

11. The apparatus of claim 1, wherein the light detector is positioned remotely from the stationary tilted intermediate image plane, and wherein the optical imaging apparatus further comprises at least one telescope that routes light from the stationary tilted intermediate image plane onto the light detector.

12. The apparatus of claim 1, wherein the light detector is positioned remotely from the stationary tilted intermediate image plane, and wherein the optical imaging apparatus further comprises a fiber-optic bundle that routes light from the stationary tilted intermediate image plane onto the light detector.

13. The apparatus of claim 1, wherein the illumination source comprises a laser that generates a pencil beam and an auxiliary light scanning element that expands the pencil beam into a sheet of light, and wherein the light detector comprises a linear image sensor.

14. The apparatus of claim 1, wherein the illumination source comprises a laser that generates a pencil beam and a cylindrical lens that expands the pencil beam into a sheet of light, and wherein the light detector comprises a 2D image sensor.

15. The apparatus of claim 1, wherein the return light comprises florescent light emitted by the tissue at the oblique imaging plane in response to illumination of the tissue at the oblique imaging plane by the illumination light.

16. An optical imaging apparatus comprising:

a beam splitter;

a light scanning element;

an objective;

an illumination source that sends illumination light into the objective via a first optical path, wherein the first optical path includes the beam splitter and the light scanning element,

wherein the beam splitter and the light scanning element redirect the illumination light towards a peripheral region of the objective such that the illumination light passes through the objective and forms an oblique imaging plane in a tissue, and wherein the position of the oblique imaging plane within the tissue varies depending on an orientation of the light scanning element,

wherein the objective accepts return light from the oblique imaging plane and passes the return light onto a second optical path that includes the beam splitter and the light scanning element, and wherein the beam splitter and the light scanning element route the return light along the second optical path so as to form a stationary tilted intermediate image plane; and

a light detector that captures an image of the stationary tilted intermediate image plane.

17. The apparatus of claim 16, wherein the light scanning element has only a single planar reflecting surface.

18. The apparatus of claim 16, wherein the illumination light arrives at the beam splitter before arriving at the light scanning element, and wherein the return light arrives at the light scanning element before arriving at the beam splitter

19. The apparatus of claim 18, further comprising a telescope disposed in both the first optical path and the second optical path, wherein the illumination light arrives at the light scanning element before arriving at the telescope, wherein the illumination light arrives at the telescope before arriving at the objective, wherein the return light arrives at the objective before arriving at the telescope, and wherein the return light arrives at the telescope before arriving at the light scanning element.

20. A method of optical imaging comprising:

sending illumination light towards an objective via a first optical path, wherein the first optical path includes a beam splitter and a first light scanning element;

redirecting the illumination light towards a peripheral region of the objective such that the illumination light passes through the objective and forms an oblique imaging plane in a tissue, wherein the position of the oblique imaging plane within the tissue varies depending on an orientation of the first light scanning element;

routing return light from the oblique imaging plane along a second optical path that includes the beam splitter and a second light scanning element, so as to form a stationary tilted intermediate image plane; and

capturing an image of the stationary tilted intermediate image plane.

21. The method of claim 20, wherein a single reflecting surface serves as both the first light scanning element and the second light scanning element.

22. The method of claim 21, wherein the illumination light arrives at the beam splitter before arriving at the single reflecting surface, and wherein the return light arrives at the single reflecting surface before arriving at the beam splitter.

23. The method of claim 20, wherein the first light scanning element and the second light scanning element use different reflecting surfaces that move in synchronization with each other.

24. The method of claim 23, wherein the illumination light arrives at the first light scanning element before arriving at the beam splitter, and wherein the return light arrives at the beam splitter before arriving at the second light scanning element.

25. The method of claim 24, wherein the first light scanning element and the second light scanning element are mechanically independent from each other.