DEFINITIVE ENDODERM CELLS AND HUMAN PLURIPOTENT STEM CELLS
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Abstract
Disclosed herein are methods of identifying one or more differentiation factors that are useful for differentiating cells in a cell population comprising definitive endoderm cells into cells which are capable of forming tissues and/or organs that are derived from the gut tube.
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Citations
39 Claims
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1-20. -20. (canceled)
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21. An in vitro method of producing human definitive endoderm cells, comprising:
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obtaining a cell population comprising pluripotent human stem cells; and providing said cell population with a TGFβ
superfamily growth factor selected from the group consisting of activn A, activin B, BMP4, nodal, and combinations thereof, thereby producing human definitive endoderm cells. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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33. An in vitro cell culture, comprising:
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human pluripotent cells; human definitive endoderm cells; and a medium comprising an effective amount of a TGFβ
superfamily member and an effective amount of a TGFβ
superfamily growth factor selected from the group consisting of activn A, activin B, BMP4, nodal and combinations thereof, wherein the effective amount of a TGFβ
superfamily growth factor selected from the group consisting of activn A, activin B, BMP4, nodal and combinations thereof promote differentiation of pluripotent cells to definitive endoderm cells. - View Dependent Claims (34, 35, 36, 37, 38)
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39. An in vitro method of producing human definitive endoderm cells, comprising:
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obtaining a cell population comprising pluripotent human stem cells; and providing said cell population with activn A, activin B and BMP4, thereby generating human definitive endoderm cells.
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Specification