FLOW-THROUGH PARAMAGNETIC PARTICLE-BASED CELL SEPARATION AND PARAMAGNETIC PARTICLE REMOVAL
First Claim
Patent Images
1. A cell processing system comprising:
- at least one cell suspension module;
at least one buffer module;
at least one flow-through magnetic separation/debeading module;
at least one non-magnetic output module; and
at least one magnetic output module.
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Accused Products
Abstract
The present disclosure relates to systems and methods for flow-through separation of paramagnetic particle-bound cells in a cell suspension containing both bound and unbound cells as well as systems and methods for removing paramagnetic particles from paramagnetic particle-bound cells or from a cell suspension with unbound cells. It further relates to a flow-through magnetic separation/debeading module and a flow-through spinning membrane debeading module.
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Citations
47 Claims
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1. A cell processing system comprising:
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at least one cell suspension module; at least one buffer module; at least one flow-through magnetic separation/debeading module; at least one non-magnetic output module; and at least one magnetic output module. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 43, 44, 45, 46, 47)
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17. A flow-through magnetic separation/debeading module comprising:
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a chamber defined by walls and having an x-direction, a y-direction, and a z-direction; an inlet and an outlet arranged on opposite ends of the chamber in the y-direction; and at least two magnets adjacent or proximate a wall of the chamber and arranged to establish a zero gradient line within the chamber between the inlet and the outlet. - View Dependent Claims (18, 19, 20, 21, 22)
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23. A spinning membrane debeading module comprising:
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a debeading chamber define partially by a cylindrical side-wall; a porous spinning membrane having an interior and oriented co-axially with the cylindrical side-wall; a sample inlet; a waste output module connected to the interior of the spinning membrane; a cell output module connected to the debeading chamber; and at least one magnet adjacent or proximate to the cylindrical side-wall. - View Dependent Claims (24, 25)
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26. A method of flow-through cell processing comprising flowing a cell suspension comprising paramagnetic particle-bound cells through a flow-through magnetic separation/debeading module to produce an unbound cell product,
wherein the paramagnetic particle-bound cells continue to move in the flow-through magnetic separation/debeading module through the flowing step, and wherein the flow-through magnetic separation/debeading module comprises: -
a flow chamber defined by walls through which the cell suspension flows; and at least two magnets arranged adjacent or proximate at least one wall. - View Dependent Claims (27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42)
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Specification