INTEGRATED TARGET WAVEGUIDE DEVICES AND SYSTEMS FOR OPTICAL COUPLING

• US 20160363728A1
• Filed: 06/11/2016
• Published: 12/15/2016
• Est. Priority Date: 06/12/2015
• Status: Active Grant

• Current Assignee: Pacific Bioscience of California Incorporated (L'Oreal SA)
• Original Assignee: Pacific Bioscience of California Incorporated (L'Oreal SA)
• Inventors: WANG, Shang, HERRMANN, Ariel, FOQUET, Mathieu, LUNDQUIST, Paul, RULISON, Aaron, MCDONALD, Mark
• Granted Patent: US 10,365,434 B2
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 21/03 Cuvette constructions
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/648 using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B
• CPC Class Codes: G01N 21/774 the reagent being on a grating or periodic structure
• CPC Class Codes: G02B 2006/12135 Temperature control
• CPC Class Codes: G02B 6/124 Geodesic lenses or integrated gratings
• CPC Class Codes: G02B 6/34 utilising prism or grating G02B6/293 takes precedence
• CPC Class Codes: G02B 6/4221 involving a visual detection of the position of the elements, e.g. by using a microscope or a camera

Abstract

Integrated target waveguide devices and optical analytical systems comprising such devices are provided. The target devices include an optical coupler that is optically coupled to an integrated waveguide and that is configured to receive optical input from an optical source through free space, particularly through a low numerical aperture interface. The devices and systems are useful in the analysis of highly multiplexed optical reactions in large numbers at high densities, including biochemical reactions, such as nucleic acid sequencing reactions. The devices provide for the efficient and reliable coupling of optical excitation energy from an optical source to the optical reactions. Optical signals emitted from the reactions can thus be measured with high sensitivity and discrimination. The devices and systems are well suited for miniaturization and high throughput.

Claims

1-73. -73. (canceled)

74. An optical analytical system comprising:

an optical source; and

an integrated target waveguide device comprising

a low numerical aperture optical coupler of at least 100 μ

m² in size; and

an integrated waveguide optically coupled to the optical coupler;

wherein the optical source is optically coupled to the optical coupler of the target waveguide device through free space at a distance of at least 1 mm.

75. The optical analytical system of claim 74, wherein the optical source has a numerical aperture of no more than 0.1.

76. The optical analytical system of claim 74, wherein the optical source is configured to illuminate a spot on the target waveguide device with a surface area per spot of at least 100 μ

m².

77. The optical analytical system of claim 74, wherein the optical source is configured to illuminate a spot on the target waveguide device with a surface area per spot of at most 250,000 μ

m².

78. The optical analytical system of claim 74, wherein the optical source is configured to illuminate a spot on the target waveguide device with a surface area per footprint of from 100 μ

m² to 250,000 μ

m².

79. The optical analytical system of claim 78, wherein the optical source is configured to illuminate the spot on the target waveguide device at a distance of from 1 mm to 100 mm.

80. The optical analytical system of claim 74, wherein the optical source is configured to illuminate a spot on the target waveguide device with a power per spot of at least 1 mW.

81. The optical analytical system of claim 80, wherein the optical source is configured to illuminate the spot on the target waveguide device at a distance of from 1 mm to 100 mm.

82. The optical analytical system of claim 74, wherein the optical source emits a plurality of light beams.

83. The optical analytical system of claim 82, wherein the optical source emits at least four light beams.

84. The optical analytical system of claim 82, wherein the optical source emits at least one sample excitation beam and at least one alignment beam.

85. The optical analytical system of claim 84, wherein the alignment beam has an alignment beam output power and the sample excitation beam has a sample beam output power, and wherein the alignment beam output power is lower than the sample excitation beam output power.

86. The optical analytical system of claim 85, wherein the alignment beam output power is no more than 10% of the sample excitation beam output power.

87. The optical analytical system of claim 84, further comprising an alignment detector.

88. The optical analytical system of claim 87, wherein the alignment detector is a camera.

89. The optical analytical system of claim 74, wherein the optical source comprises a planar lightwave circuit.

90. The optical analytical system of claim 74, wherein the optical source provides light of wavelength in the range from 450 nm to 650 nm.

91. The optical analytical system of claim 74, further comprising a heat sink.

92. The optical analytical system of claim 74, wherein the integrated target waveguide device is removable.

93. The optical analytical system of claim 74, further comprising an optical element, wherein the optical element is positioned between the optical source and the target waveguide device.

94. The optical analytical system of claim 93, wherein the optical element modulates the focus of an optical beam transmitted from the optical source to the target waveguide device.

95. The optical analytical system of claim 93, wherein the optical element modulates the size of the footprint of an optical beam transmitted from the optical source to the target waveguide device.

96. The optical analytical system of claim 74, wherein the optical coupler of the target waveguide device has a numerical aperture of no more than 0.10.

97. The optical analytical system of claim 74, wherein the optical coupler of the target waveguide device is a grating coupler.

98. The optical analytical system of claim 74, wherein the target waveguide device further comprises a reflective layer positioned below the optical coupler.

99. The optical analytical system of claim 74, wherein the target waveguide device further comprises a heat spreading layer in thermal contact with the optical coupler.

100. The optical analytical system of claim 74, wherein the target waveguide device further comprises an alignment feature.

101. The optical analytical system of claim 74, wherein the target waveguide device comprises a plurality of optical couplers and a plurality of integrated waveguides optically coupled to the plurality of optical couplers.

102. The optical analytical system of claim 74, wherein the target waveguide device further comprises a plurality of nanoscale sample wells optically coupled to the integrated waveguide.