ION EXCHANGE CHROMATOGRAPHY COLUMN, METHOD, AND SYSTEM THEREOF
First Claim
1. A chromatography column, the column containing a packed bed of an ion exchange chromatographic packing material, the column comprising:
- a) support resin particles; and
b) a copolymer comprising polymerized functional monomers that include a first ion exchange group monomer and a second ion exchange group monomer, where the first ion exchange group monomer and the second ion exchange group monomer are different,in which the first ion exchange group monomer is configured to have a first charge at a first pH, and the second ion exchange group monomer is configured to have a net neutral charge at the first pH,in which the first ion exchange group monomer is configured to have the first charge at a second pH, and the second ion exchange group monomer is configured to have a net neutral charge or a second charge at the second pH where the first charge and second charge both have a same polarity, in which the first pH and the second pH are different,in which a mole percent of the second ion exchange group monomer is a mole amount of the second ion exchange group monomer divided by a summation of a mole amount of the first ion exchange group monomer and the mole amount of the second ion exchange group monomer, and multiplied by 100, the mole percent of the second ion exchange group monomer ranges from about 60% to about 99%,in which the copolymer is grafted to the support resin particles.
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Abstract
An ion exchange chromatographic packing material is described that includes support resin particles and a copolymer grafted to the support resin particles. The copolymer includes polymerized functional monomers such as a first ion exchange group monomer and a second ion exchange group monomer. At a first pH, the first ion exchange group monomer is configured to have a first charge at a first pH, and the second ion exchange group monomer is configured to have a net neutral charge. At a second pH, the first ion exchange group monomer is configured to have the first charge at a second pH, and the second ion exchange group monomer is configured to have a second charge at the second pH where the first charge and second charge both have a same polarity.
6 Citations
26 Claims
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1. A chromatography column, the column containing a packed bed of an ion exchange chromatographic packing material, the column comprising:
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a) support resin particles; and b) a copolymer comprising polymerized functional monomers that include a first ion exchange group monomer and a second ion exchange group monomer, where the first ion exchange group monomer and the second ion exchange group monomer are different, in which the first ion exchange group monomer is configured to have a first charge at a first pH, and the second ion exchange group monomer is configured to have a net neutral charge at the first pH, in which the first ion exchange group monomer is configured to have the first charge at a second pH, and the second ion exchange group monomer is configured to have a net neutral charge or a second charge at the second pH where the first charge and second charge both have a same polarity, in which the first pH and the second pH are different, in which a mole percent of the second ion exchange group monomer is a mole amount of the second ion exchange group monomer divided by a summation of a mole amount of the first ion exchange group monomer and the mole amount of the second ion exchange group monomer, and multiplied by 100, the mole percent of the second ion exchange group monomer ranges from about 60% to about 99%, in which the copolymer is grafted to the support resin particles. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 25, 26)
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19. A chromatography column, the column containing a packed bed of an ion exchange chromatographic packing material, the ion exchange chromatographic packing material made by a method comprising:
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mixing a first ion exchange group monomer, a second ion exchange group monomer, and an initiator in an aqueous liquid with support resin particles to form a reaction mixture, where the first ion exchange group monomer and the second ion exchange group monomer are different, in which the reaction mixture is at a first pH where the first ion exchange group monomer has a first charge and the second ion exchange group monomer has a net neutral charge; polymerizing the reaction mixture to form a copolymer that is grafted to the support resin particles, the copolymer comprising polymerized functional monomers that include the first ion exchange group monomer and the second ion exchange group monomer; and in which the second ion exchange group monomer of the grafted support has a second charge where the first charge and the second charge have a same polarity at a second pH, in which the first pH and the second pH are different. - View Dependent Claims (20, 21, 22, 23, 24)
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Specification