IMAGING DEVICE FOR MICROSCOPE

• US 20160377852A1
• Filed: 09/06/2016
• Published: 12/29/2016
• Est. Priority Date: 03/06/2014
• Status: Active Grant

• Current Assignee: ACQUIFER Imaging GmbH (Bruker Corporation), Karlsruher Institut Fur Technologie
• Original Assignee: Acquifer Ag, European Molecular Biology Laboratory, Karlsruher Institut Fur Technologie
• Inventors: Liebel, Urban, Hufnagel, Lars, Krzic, Uros
• Granted Patent: US 10,642,018 B2
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: G02B 21/06 Means for illuminating specimens
• CPC Class Codes: G02B 21/16 adapted for ultraviolet illumination ; Fluorescence microscopes G02B21/0076 takes precedence
• CPC Class Codes: G02B 21/26 Stages; Adjusting means therefor
• CPC Class Codes: G02B 21/28 with cooling device
• CPC Class Codes: G02B 21/362 Mechanical details, e.g. mountings for the camera or image sensor, housings G02B21/364 takes precedence

Abstract

An imaging device for a microscope (20) is disclosed that comprises a plurality of individual light sources (40) and a detector (15). The plurality of the individual light sources (40) is mounted on a translation stage (110). The imaging device further includes moveable optics arranged to direct a light path (45) onto a stationary sample (60) and collect reflected or fluoresced radiation from the sample (60).

Claims

1. An imaging device for imaging at least one stationary sample comprising:

a plurality of individual light sources for illuminating the at least one stationary sample;

a detector for detection of radiation from the at least one stationary sample; and

an optical arrangement arranged such that a light path from at least one of the plurality of individual light sources illuminates the at least one stationary sample and directs the radiation from the at least one stationary sample to the detector.

2. The imaging device of claim 1, further comprising a Peltier device arranged between at least one of the plurality of individual light sources and heat sink on which the plurality of individual light sources is mounted.

3. The imaging device of claim 1, further comprising a band pass filter arranged in the light path between the at least one of the plurality of individual light sources and the stationary sample.

4. The imaging device of claim 3, further comprising a diffuser being arranged in the light path between the band pass filter and the stationary sample.

5. The imaging device of claim 1, further comprising a motor to move the individual light sources with respect to the detector.

6. The imaging device of claim 1, wherein the plurality of individual light sources is movable with respect to a fixed objective lens.

7. The imaging device of claim 1, wherein the individual light sources are light emitting diodes.

8. A microscope for imaging at least one stationary sample comprising:

an objective lens;

a translation stage on which the imaging device of claim 1 is mounted; and

an immoveable sample holder on which the at least one stationary sample is mounted.

9. The microscope of claim 8, further comprising a plurality of translation rails cooperating with at least one motor to move the imaging device with respect to the stationary sample.

10. The microscope of claim 8, wherein the imaging device is movable in two dimensions.

11. The microscope of claim 8, wherein the plurality of individual light sources is movable with respect to the objective lens such that different ones of the plurality of individual light sources illuminate the stationary sample.