MICROORGANISMS HAVING PUTRESCINE PRODUCTIVITY AND PROCESS FOR PRODUCING PUTRESCINE USING THE SAME

• US 20170002386A1
• Filed: 02/25/2014
• Published: 01/05/2017
• Est. Priority Date: 03/20/2013
• Status: Active Grant

• Current Assignee: CJ Cheiljedang Corporation (CJ Group)
• Original Assignee: CJ Cheiljedang Corporation (CJ Group)
• Inventors: JUNG, Hee Kyoung, LI, Hong Xian, PARK, Su Jin, YOON, Jong Hyun, LEE, Baek Seok, LEE, Sun Young, LEE, Kyoung Min, YANG, Young Lyeol, UM, Hye Won, KIM, Chang Gyeom
• Granted Patent: US 10,221,433 B2
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: C12N 15/52 Genes encoding for enzymes or proenzymes
• CPC Class Codes: C12N 9/0008 acting on the aldehyde or oxo group of donors (1.2)
• CPC Class Codes: C12N 9/1018 Carboxy- and carbamoyl transferases (2.1.3)
• CPC Class Codes: C12N 9/1029 transferring groups other than amino-acyl groups (2.3.1)
• CPC Class Codes: C12N 9/1096 transferring nitrogenous groups (2.6)
• CPC Class Codes: C12N 9/1217 Phosphotransferases with a carboxyl group as acceptor (2.7.2)
• CPC Class Codes: C12N 9/88 Lyases (4.)
• CPC Class Codes: C12P 13/001 Amines; Imines
• CPC Class Codes: C12Y 102/01038 N-Acetyl-gamma-glutamyl-phosphate reductase (1.2.1.38)
• CPC Class Codes: C12Y 201/03003 Ornithine carbamoyltransferase (2.1.3.3)
• CPC Class Codes: C12Y 203/01035 Glutamate N-acetyltransferase (2.3.1.35)
• CPC Class Codes: C12Y 206/01011 Acetylornithine transaminase (2.6.1.11)
• CPC Class Codes: C12Y 207/02008 Acetylglutamate kinase (2.7.2.8)
• CPC Class Codes: C12Y 401/01007 Benzoylformate decarboxylase (4.1.1.7)

Abstract

The present invention relates to a recombinant microorganism capable of producing putrescine, in which the microorganism is modified to have enhanced NCgl2522 activity, thereby producing putrescine in a high yield, and a method for producing putrescine using the microorganism.

Claims

1. A microorganism having putrescine productivity, which is modified to have enhanced activity of a protein having an amino acid sequence represented by SEQ ID NO:

21 or 23.

2. The microorganism having putrescine productivity according to claim 1, wherein the microorganism is further modified to have weakened activities of ornithine carbamoyltransferase (ArgF) and a protein (NCgl1221) involved in glutamate export, compared to the endogenous activities, and to have enhanced ornithine decarboxylase (ODC) activity.

3. The microorganism having putrescine productivity according to claim 2, wherein the ornithine carbamoyltransferase (ArgF) has an amino acid sequence represented by SEQ ID NO:

29, the protein (NCgl1221) involved in glutamate export has an amino acid sequence represented by SEQ ID NO;

30, and the ornithine decarboxylase (ODC) has an amino acid sequence represented by SEQ ID NO;

33.

4. The microorganism having putrescine productivity according to claim 1, wherein the microorganism is further modified to have enhanced activities of acetyl-gamma-glutamyl-phosphate reductase (ArgC), acetylglutamate synthase or ornithine acetyltransferase (ArgJ), acetylglutamate kinase (ArgB), and acetylornithine aminotransferase (ArgD), compared to the endogenous activities.

5. The microorganism having putrescine productivity according to claim 4, wherein the acetyl-gamma-glutamyl-phosphate reductase (ArgC), acetylglutamate synthase or ornithine acetyltransferase (ArgJ), acetyl glutamate kinase (ArgB), and acetylornithine aminotransferase (ArgD) have amino acid sequences represented by SEQ ID NOs:

25, 26, 27 and 28, respectively.

6. The microorganism having putrescine productivity according to claim 1, wherein acetyltransferase (NCgl1469) activity of the microorganism is further weakened.

7. The microorganism having putrescine productivity according to claim 6, wherein the acetyltransferase (NCgl1469) has an amino acid sequence represented by SEQ ID NO:

31 or 32.

8. The microorganism having putrescine productivity according to claim 1, wherein the microorganism is an Escherichia sp. or a Corynebacterium sp.

9. The microorganism having putrescine productivity according to claim 8, wherein the microorganism is E. coli or Corynebacterium glutamicum.

10. A method for producing putrescine, comprising the steps of:

(i) culturing the microorganism having putrescine productivity of any one of claims 1 to 9 to obtain a cell culture; and

(ii) recovering putrescine from the cultured microorganism or the cell culture.