Sequencing Method Using Magnifying Tags

• US 20170016061A1
• Filed: 06/17/2016
• Published: 01/19/2017
• Est. Priority Date: 12/23/1998
• Status: Active Application

• Current Assignee: Empire IP LLC
• Original Assignee: Empire IP LLC
• Inventors: Lexow, Preben
• Application Number:
  US15/186,372
• Publication Number:
  US 20170016061A1
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: C12Q 2521/313 Type II endonucleases, i.e. cutting outside recognition site
• CPC Class Codes: C12Q 2521/501 Ligase
• CPC Class Codes: C12Q 2525/155 incorporating/generating a new priming site
• CPC Class Codes: C12Q 2525/185 incorporating bases where the precise position of the bases in the nucleic acid string is important
• CPC Class Codes: C12Q 2525/191 incorporating an adaptor

Abstract

The present invention provides a method of sequencing all or part of a target nucleic acid molecule by determining the sequence of a portion of said nucleic acid molecule in addition to information regarding the position of said portion and in particular provides a new method of sequencing involving the magnification of one or more bases of said bases to aid identification.

Claims

1-25. -25. (canceled)

26. A molecule for magnifying a signal associated with at least one base in a target nucleic acid molecule, comprising:

an adapter for hybridizing to at least one base in a single strand of a target nucleic acid molecule, wherein the adapter includes at least one complementary base for the at least one base;

a magnifying tag joined to the adapter, wherein the magnifying tag comprises a tag adapted to produce a magnified signal indicative of a characteristic of the at least one complementary base, wherein the characteristic is at least one of a size and a spatial configuration.

27. The molecule according to claim 26, wherein the adapter includes a cleavage site.

28. The molecule according to claim 26, further comprising a link portion adapted to couple to an adjacent adapter hybridized to the single strand.

29. A kit for magnifying a signal associated with at least one base in a target nucleic acid molecule:

at least four molecules for magnifying signals associated with respective bases in a target nucleic acid molecule, wherein each of the at least four molecules comprises;

an adapter for hybridizing to a respective base in a single strand of the target nucleic acid molecule, wherein the adapter includes a complementary base for the respective base; and

a magnifying tag joined to the adapter, wherein the magnifying tag comprises a tag adapted to produce a magnified signal indicative of a characteristic of the complementary base, wherein the characteristic is at least one of a size and a spatial configuration,

an enzyme adapted to join adjacent ones of the at least four molecules to form a complimentary strand to the single strand and to join the complimentary strand to the single strand to form a double-strand; and

a nanopore adapted to receive the complimentary strand when separated from the double strand and adapted to generate the magnified signal based on the tag.