AGRICULTURAL ENDOPHYTE-PLANT COMPOSITIONS, AND METHODS OF USE
First Claim
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1. A method for preparing an agricultural seed preparation, comprising:
- a) contacting the surface of a plurality of Glycine agricultural plant seeds with a formulation comprising a purified bacterial population at a concentration of at least about 102 CFU/ml in a liquid formulation or about 102 CFU/gm in a non-liquid formulation, the bacterial population comprising a seed bacterial endophyte exhibiting the ability to produce or induce in a Glycine agricultural plant grown from the Glycine agricultural plant seed an activity selected from production of an auxin, production of an antimicrobial, production of a siderophore, mineral phosphate solubilization, production of a cellulase, production of a chitinase, production of a xylanase, and production of acetoin, wherein the seed bacterial endophyte is present in the formulation in an amount capable of providing a benefit to the plant seeds or to agricultural plants derived from the plant seeds, wherein the formulation further comprises a stabilizer, a fungicide, a preservative, or a combination thereof; and
b) packaging the contacted seeds in a container.
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Abstract
This application relates to methods and materials for providing a benefit to a seed or seedling of an agricultural plant (e.g., an agricultural grass plant or a Gycine plant), or the agricultural plant derived from the seed or seedling. For example, this application provides purified bacterial populations that include novel seed bacterial endophytes, and synthetic combinations of seeds and/or seedlings (e.g., cereal or soy seeds and/or cereal or soy seedlings) with heterologous seed-derived bacterial endophytes.
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Citations
59 Claims
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1. A method for preparing an agricultural seed preparation, comprising:
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a) contacting the surface of a plurality of Glycine agricultural plant seeds with a formulation comprising a purified bacterial population at a concentration of at least about 102 CFU/ml in a liquid formulation or about 102 CFU/gm in a non-liquid formulation, the bacterial population comprising a seed bacterial endophyte exhibiting the ability to produce or induce in a Glycine agricultural plant grown from the Glycine agricultural plant seed an activity selected from production of an auxin, production of an antimicrobial, production of a siderophore, mineral phosphate solubilization, production of a cellulase, production of a chitinase, production of a xylanase, and production of acetoin, wherein the seed bacterial endophyte is present in the formulation in an amount capable of providing a benefit to the plant seeds or to agricultural plants derived from the plant seeds, wherein the formulation further comprises a stabilizer, a fungicide, a preservative, or a combination thereof; and b) packaging the contacted seeds in a container. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 23, 24)
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17-22. -22:
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25-46. -46. (canceled)
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47. A method of making a seed treatment, the method comprising:
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(a) inoculating an isolated cultured bacterial endophyte selected from Table 1 into a liquid medium; (b) culturing the bacterial endophyte in the medium at a temperature of about 4 to about 37 degrees C. until the cell or spore density in the medium is at least about 102 CFU/mL; and (c) collecting the vegetative cells or spores under conditions suitable to produce a seed treatment.
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48-57. -57. (canceled)
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58. A method of screening a modulator of a Glycine trait, comprising:
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(a) applying a library of bacterial entities to a population of seeds of a Glycine agricultural plant; (b) measuring a trait in seedlings or plants derived from the seeds, the trait selected from the group consisting of root biomass, root length, height, shoot length, leaf number, water use efficiency, overall biomass, yield, photosynthesis rate, tolerance to drought, heat tolerance, salt tolerance, resistance to nematode stress, resistance to a fungal pathogen, resistance to a bacterial pathogen, resistance to a viral pathogen, the level of a metabolite, and proteome expression; and (c) identifying at least one of the bacterial entities present in the library capable of modulating the trait, relative to reference seedlings or plants.
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59-150. -150. (canceled)
Specification