METHOD AND APPARATUS FOR ACQUIRING BLOOD FOR TESTING

• US 20170023446A1
• Filed: 02/19/2016
• Published: 01/26/2017
• Est. Priority Date: 02/20/2015
• Status: Abandoned Application

• Current Assignee: Neoteryx, LLC
• Original Assignee: Neoteryx, LLC
• Inventors: Rietveld, Gijsbertus G., Kushon, Stuart, Kansal, Monika M.
• Application Number:
  US15/048,859
• Publication Number:
  US 20170023446A1
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: A61B 5/150358 Strips for collecting blood, e.g. absorbent optical reagent test strips G01N21/8483; chemical reagent test strips G01N33/4875, G01N33/52
• CPC Class Codes: A61B 5/150755 Blood sample preparation for further analysis, e.g. by separating blood components or by mixing
• CPC Class Codes: B01L 2300/12 Specific details about materials
• CPC Class Codes: B01L 3/5029 using swabs
• CPC Class Codes: B01L 9/543 for disposable pipette tips, e.g. racks or cassettes
• CPC Class Codes: G01N 1/10 in the liquid or fluent state burettes, pipettes B01L3/02; sampling of ground water E02D1/06; metering by volume of fluids or fluent solid material G01F11/00, G01F13/00
• CPC Class Codes: G01N 2001/1056 Disposable (single-use) samplers
• CPC Class Codes: G01N 33/49 Blood chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80

Abstract

A blood sampling device is provided having holder with a manipulating end and an absorbent probe on the opposing end. The probe is made of pyrolyzed porous carbon sized to directly absorb a predetermined volume of liquid, preferably biological fluid, in a predetermined amount of time. Shapes for absorbent probes of differing materials are provided.

Claims

1. A biological fluid sampling device, comprising:

an absorbent probe made of an open cell, porous carbonized material; and

a holder connected to the probe and configured to allow a user to manually manipulate the holder and probe during use.

2. The device of claim 1, wherein the absorbent probe is of sufficient size to absorb for analysis about 1 μ

l to about 100 μ

l of blood in about 2-5 seconds without separating the blood from plasma, the probe having a length of less than about 5 mm and a cross-sectional area of less than about 20 mm² with a majority of the exterior surface of the probe being exposed and available for placing against a fluid sample on a surface to absorb the sample;

3. The device of claim 2, wherein the probe is made of pyrolyzed carbon with about 90% or more of the absorbent probe made of carbon, by weight.

4. The device of claim 2, wherein the probe is made of pyrolyzed carbon with about 95% or more of the absorbent probe made of carbon, by weight.

5. The device of claim 3, wherein the holder is dimensionally suited for use with devices that can manipulate a pipette tip.

6. The device of claim 2, wherein the holder is disposable and the probe has a pore volume of about 35% or more.

7. The device of claim 3, wherein the size is sufficient to absorb about 20 microliters.

8. The device of claim 3, wherein the probe has a cylindrical portion that is sized to fit into a mating opening in the holder to connect the holder to the probe.

9. The device of claim 3, wherein the probe and holder are sterile and packaged in a sterile container.

10. The device of claim 2, wherein the probe contains dried anti-coagulant.

11. The device of claim 3, wherein the probe contains dried anti-coagulant.

12. The device of claim 2, wherein the probe contains at least one of a reference standard, a dried stabilizer or a modifier.

13. The device of claim 2, wherein the probe contains dried blood.

14. The device of claim 3, wherein the probe contains dried blood.

15. The device of claim 3, further comprising a container having a recess configured to enclose the holder for transportation of the holder.

16. The device of claim 15, wherein the container has a plurality of openings allowing air to access the probe.

17. A process for use in testing a blood sample, comprising:

placing an absorbent probe in physical contact with a blood sample, the absorbent probe being made of a carbonized, porous carbon material and connected to a holder, the absorbent probe being configured to absorb a predetermined maximum volume of blood of about 1 μ

l to about 100 μ

l, the absorbent probe having a majority of its exterior surface exposed and accessible for absorption of blood from a surface;

maintaining a portion of the exterior surface of the probe in contact with the blood sample until the predetermined amount of blood is absorbed by the probe;

removing the probe from contact with the blood; and

drying the blood in the probe without contaminating the blood.

18. The process of claim 17, wherein the absorbent probe is made of pyrolyzed carbon with about 90% or more of the absorbent probe made of carbon.

19. The process of claim 17, wherein the absorbent probe is made of pyrolyzed carbon with about 95% or more of the absorbent probe made of carbon, by weight.

20. The process of claim 18, wherein the predetermined time is less than about five seconds.

21. The process of claim 18, wherein the blood sample is on a live animal when contacted by the probe.

22. The process of claim 18, wherein the probe is configured to absorb a predetermined amount of blood of about 5-15 microliters.

23. The process of claim 18, wherein said majority of the exterior surface of the probe has a porosity of about 30% to 50%.

24. The process of claim 18, further comprising placing the probe with the dried blood in a compartment within a container.

25. The process of claim 18, further comprising placing the probe with dried blood in a container along with a fluid selected to reconstitute the dried blood in the probe.

26. The process of claim 18, comprising a plurality of probes each held in a pipette tip and each containing dried blood, the pipette tips being held in a tray.

27. A kit for collecting body fluids, comprising:

a plurality of holders each having a manipulating end and opposite thereto an absorbent probe made of a hydrophilic, porous carbon material configured to absorb a predetermined volume of about 1 μ

l to about 100 μ

l of blood within about 1-5 seconds, the probe having a majority of its exterior surface exposed for potential use in absorbing the blood;

a container having a plurality of compartments, each configured to releasably receive a different one of the holders and its probe, the container and holder configured to prevent the probes from abutting the compartment within which the holder and probe are placed, the container having openings in each compartment to allow air to enter each of the compartments and reach the probe within the compartment with which the openings are associated.

28. The kit of claim 27, wherein the absorbent probe is made of pyrolyzed carbon with about 90% or more of the absorbent probe made of carbon, by weight.

29. The kit of claim 27, wherein the absorbent probe is made of pyrolyzed carbon with about 95% or more of the absorbent probe made of carbon, by weight.

30. The kit of claim 28, further comprising a plurality of access ports with each port associated with a different one of the compartments, each port located to allow printing onto the manipulating end of the holder in the compartment with which the port is associated.

31. The kit of claim 28, wherein the container has two parts cooperating to form tubular compartments containing different ones of the absorbent probes.

32. The kit of claim 28, wherein the probes are configured to absorb about 1-7 microliters of blood.

33. The kit of claim 28, wherein at least one of the probes contains dried blood.

34. The kit of claim 28, wherein at least one of the probes contains a dried anticoagulant.

35. The kit of claim 28, wherein at least one of the compartments contains a desiccant.

36. A method of forming an absorbent probe for absorbing liquids, comprising:

pyrolyzing a high carbon precursor to form a porous, open cell, carbonized probe;

fastening the probe to a holder configured to allow a user to manually manipulate the probe and holder during use.

37. The method of claim 36, wherein the carbonized probe has about 95% or more of the absorbent probe made of carbon, by weight.

38. The method of claim 36, wherein the carbonized probe has about 95% or more of the absorbent probe made of carbon, by weight.

39. The method of claim 37, further comprising absorbing an anticoagulant into the absorbent probe.

40. The method of claim 36, wherein the carbonized probe has a porosity of about 40%.

41. A fluid collection device, comprising:

a holder having a body configured to be gripped and manipulated by a person'"'"'s hand and having a distal end with distal tip thereon, the holder having a longitudinal axis extending along a length of the holder;

an absorbent probe connected to the distal tip, the distal tip having a sidewall and end forming a continuous surface with the sidewall of the absorbent probe encircling a portion of the distal tip and the end of the absorbent probe forming a closed bottom of the recess, the absorbent probe end and sidewall having a substantially uniform thickness and an exterior surface with no concave portions thereon.

42. The fluid collection device of claim 41, wherein the absorbent probe is made of porous carbon.

43. The fluid collection device of claim 41, wherein the absorbent probe is made of sintered plastic.

44. The fluid collection device of claim 41, wherein the absorbent probe has a bullet shape with generally parallel sidewalls and a hemi-spherical end.

45. The fluid collection device of claim 41, wherein the absorbent probe has a conical shape.

46. The fluid collection device of claim 41, wherein the absorbent probe has a bullet shape with generally parallel sidewalls and a curved end having a conical surface forming at least a portion of that end.

47. The fluid collection device of claim 41, wherein the absorbent tip comprises absorbent means configured for absorbing a predetermined volume of fluid.

48. The fluid collection device of claim 41, wherein the sidewall of the absorbent tip has a thickness of about 0.015 to 0.063 inches.

49. The fluid collection device of claim 41, wherein the sidewall of the absorbent probe has a thickness of about 0.015 inches.

50. The fluid collection device of claim 43, wherein the absorbent probe comprises a non-porous inner portion connected to the distal tip and a porous outer portion.