DNA POLYMERASES FROM THE RED SEA BRINE POOL

• US 20170029793A1
• Filed: 04/10/2015
• Published: 02/02/2017
• Est. Priority Date: 04/11/2014
• Status: Active Grant

• Current Assignee: King Abdullah University of Science and Technology
• Original Assignee: King Abdullah University of Science and Technology
• Inventors: Hamdan, Samir M., Takahashi, Masateru
• Granted Patent: US 10,443,048 B2
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: C12N 9/1252 DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
• CPC Class Codes: C12P 19/34 Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• CPC Class Codes: C12Y 207/07007 DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Abstract

A DNA polymerase composition for amplifying nucleic acids can be tolerant of extreme conditions.

Claims

1. A DNA polymerase composition for amplifying nucleic acids comprising:

an isolated DNA polymerase having at least 80% homology with the sequence of SEQ ID NO;

1-4.

2. A method for amplifying nucleic acid comprising:

reacting DNA as a template, a primer, dNTP and the DNA polymerase composition of claim 1, and extending the primer to synthesize a DNA primer extension product.

4. A vector comprising a gene encoding the DNA polymerase of claim 1.

6. The DNA polymerase composition of claim 1 wherein the isolated DNA polymerase has at least 80% homology with the sequence of SEQ ID NO:

1.

7. The DNA polymerase composition of claim 1 wherein the isolated DNA polymerase has at least 80% homology with the sequence of SEQ ID NO:

2.

8. The DNA polymerase composition of claim 1 wherein the isolated DNA polymerase has at least 80% homology with the sequence of SEQ ID NO:

3.

9. The DNA polymerase composition of claim 1 wherein the isolated DNA polymerase has at least 80% homology with the sequence of SEQ ID NO:

4.

3. A kit for amplifying nucleic acid comprising the DNA polymerase composition of claim

5. A method of utilizing the Brine pool to isolate a DNA polymerase,

wherein the polymerase retains its activity in the presence of high salts and metal ions concentrations and different type of metal ions including Zn²⁺.