RNA-INTERFERENCE BY SINGLE-STRANDED RNA MOLECULES
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Abstract
The present invention relates to sequence and structural features of single-stranded (ss)RNA molecules required to mediate target-specific nucleic acid modifications by RNA-interference (RNAi), such as target mRNA degradation and/or DNA methylation.
13 Citations
39 Claims
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1-19. -19. (canceled)
- 20. Purified human RISC, wherein said RISC is a ribonucleoprotein complex containing a single stranded RNA component, and wherein said RNA component comprises at least one modified nucleotide analogue which is selected from sugar backbone and nucleobase-modified ribonucleotides and combinations thereof.
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39. A method of mediating RNA interference in a cell or an organism, comprising
(a) contacting a cell or organism with a purified human RISC having a molecular weight of less than about 160 kDa, wherein said RISC is a ribonucleoprotein complex containing a single stranded RNA component, and wherein said RNA component comprises at least one modified nucleotide analogue which is selected from sugar backbone and nucleobase-modified ribonucleotides and combinations thereof, under conditions where target specific nucleic acid modifications can occur; - and
(b) mediating a target specific nucleic acid modification effected by the single stranded RNA towards a target nucleic acid having a sequence portion substantially complementary to the single stranded RNA.
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Specification