METHODS FOR DIAGNOSING INFECTIOUS DISEASES USING ADSORPTION MEDIA
First Claim
1. An in vitro method for concentrating a pathogen present in a biological sample obtained from a subject who is suspected of being infected with said pathogen, wherein said pathogen comprises a bacteria of genus Borrelia, the method comprising:
- (a) contacting the biological sample obtained from the subject with an adsorption media under conditions to form an adhering complex comprising the adsorption media and said pathogen;
(b) separating the adhering complex from components of the sample that are not included in the complex while maintaining the complex;
(c) washing the adhering complex with a wash buffer; and
(d) collecting pathogens of the adhering complex by applying an elution buffer to the complex, thereby concentrating the pathogen in an eluent.
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Abstract
The present invention provides an in vitro method for concentrating infectious pathogens found in a biological sample obtained from an individual who is suspected of being infected with the pathogens. Provided herein is also an in vitro method for reducing or eliminating blood cells from a sample obtained from an individual suspected to being infected with an infectious pathogen. The present invention also provides a method for diagnosing malaria and a method for determining if an individual is infected with a pathogen. Provided herein is also a concentrator and a kit for use with the methods.
13 Citations
24 Claims
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1. An in vitro method for concentrating a pathogen present in a biological sample obtained from a subject who is suspected of being infected with said pathogen, wherein said pathogen comprises a bacteria of genus Borrelia, the method comprising:
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(a) contacting the biological sample obtained from the subject with an adsorption media under conditions to form an adhering complex comprising the adsorption media and said pathogen; (b) separating the adhering complex from components of the sample that are not included in the complex while maintaining the complex; (c) washing the adhering complex with a wash buffer; and (d) collecting pathogens of the adhering complex by applying an elution buffer to the complex, thereby concentrating the pathogen in an eluent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A method for extracorporeally removing an infection caused by a bacteria of genus Borrelia from a subject to treat Lyme disease, the method comprising:
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(a) contacting a blood sample obtained from said subject with an adsorption media under conditions to form an adhering complex comprising the adsorption media and a cell present in the blood sample which is infected with the bacteria; and (b) removing the adhering complex from the blood sample to treat Lyme disease. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24)
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Specification