ELECTRIC PULSE GENERATOR FOR ELECTROPORATOR AND ELECTROPORATOR APPARATUS PROVIDED WITH ELECTRIC PULSE GENERATOR

• US 20170080222A1
• Filed: 12/06/2016
• Published: 03/23/2017
• Est. Priority Date: 07/28/2014
• Status: Active Grant

• Current Assignee: Nepa Gene Co., Ltd.
• Original Assignee: Nepa Gene Co., Ltd.
• Inventors: Hayakawa, Yasuhiko, Hayakawa, Kiyoshi, Sugiyama, Masahiko, AYUSAWA, Dai, MIKI, Kensuke
• Granted Patent: US 10,004,899 B2
• Time in Patent Office: Days
• CPC Class Codes: A61N 1/30 Apparatus for iontophoresis, i.e. transfer of media in ionic state by an electromotoric force into the body, or cataphoresis
• CPC Class Codes: A61N 1/327 for enhancing the absorption properties of tissue, e.g. by electroporation
• CPC Class Codes: C12M 35/02 Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
• CPC Class Codes: C12N 15/09 Recombinant DNA-technology
• CPC Class Codes: H03K 3/00 Circuits for generating electric pulses; Monostable, bistable or multistable circuits H03K4/00 takes precedence; for digital function generators in computers G06F1/02
• CPC Class Codes: H03K 3/53 by the use of an energy-accumulating element discharged through the load by a switching device controlled by an external signal and not incorporating positive feedback H03K3/335 takes precedence

Abstract

An electric pulse generator for electroporator includes a poring pulse generator, and a transfer pulse generator. The poring generator includes an n-stage Cockroft-Walton circuit, and a first circuit branched from a branching point of wiring on output side of the Cockroft-Walton, the first circuit includes a switching switch that is turned off in high-voltage mode and turned on in low-voltage mode, a voltage value for switching between the modes is in 200 to 1400 V, the first circuit includes a second circuit in which m₂ series of m₁ series-connected capacitors are connected in parallel, the switch and second circuit are connected in series, and first wiring on output side of the second circuit is merged with second wiring on output side of the Cockroft-Walton on output side of the branching point, and a withstand voltage of the Cockroft-Walton is a value in 1500 to 5000 V in the high-voltage mode.

Claims

1. An electric pulse generator for an electroporator, comprising:

a poring pulse generator; and

a transfer pulse generator,

wherein the poring pulse generator comprises an n-stage Cockroft-Walton circuit, where n represents an integer of 2 or more, and a first circuit that is branched from a branching point of wiring on an output side of the n-stage Cockroft-Walton circuit, the first circuit includes a switching switch that is turned off in a high-voltage mode and turned on in a low-voltage mode, a voltage value for switching between the high-voltage mode and the low-voltage mode being in a range of 200 to 1400 V, the first circuit includes a second circuit in which m₂ series of m₁ series-connected capacitors are connected in parallel, where m₁ represents an integer of 1 or more, and m₂ represents an integer of 2 or more, the switching switch and the second circuit are connected in series, and first wiring, which is disposed on an output side of the second circuit, is merged with second wiring, which is disposed on the output side of the n-stage Cockroft-Walton circuit, on the output side of the branching point, and a withstand voltage of the n-stage Cockroft-Walton circuit is a value in a range of 1500 to 5000 V in the high-voltage mode.

2. The electric pulse generator according to claim 1, wherein the first circuit includes a diode on an output side of the second circuit in the first circuit, the diode being connected in a direction in which current flows toward the output side.

3. The electric pulse generator according to claim 1, wherein the capacitors of the second circuit have a withstand voltage of 500 V or lower.

4. The electric pulse generator according to claim 1, wherein the voltage value for switching between the high-voltage mode and the low-voltage mode is a voltage value in a range of 500 to 1000 V.

5. The electric pulse generator according to claim 1, wherein the poring pulse generator includes only one set of a poring pulse generating direct-current power source and a capacitor charging circuit, and a total electric capacity of the n-stage Cockroft-Walton circuit and the second circuit is any value in a range of 500 to 4000 μ

F in the low-voltage mode.

6. The electric pulse generator according to claim 1, wherein the integer n in the n-stage Cockroft-Walton circuit is any integer in a range of 4 to 13, the integer m₁ in the second circuit is any integer in a range of 2 to 6, and the integer m₂ in the second circuit is any integer in a range of 2 to 10.

7. The electric pulse generator according to claim 1, further comprising:

at least one polarity switching circuit configured to control switching of a polarity of output voltage.

8. The electric pulse generator according to claim 1, further comprising:

at least one pulse control circuit configured to control an output pulse.

9. An electroporator apparatus including the electric pulse generator according to claim 1.

10. The electric pulse generator according to claim 2, wherein the capacitors of the second circuit have a withstand voltage of 500 V or lower.

11. The electric pulse generator according to claim 2, wherein the voltage value for switching between the high-voltage mode and the low-voltage mode is a voltage value in a range of 500 to 1000 V.

12. The electric pulse generator according to claim 2, wherein the poring pulse generator includes only one set of a poring pulse generating direct-current power source and a capacitor charging circuit, and a total electric capacity of the n-stage Cockroft-Walton circuit and the second circuit is any value in a range of 500 to 4000 μ

F in the low-voltage mode.

13. The electric pulse generator according to claim 2, wherein the integer n in the n-stage Cockroft-Walton circuit is any integer in a range of 4 to 13, the integer m₁ in the second circuit is any integer in a range of 2 to 6, and the integer m₂ in the second circuit is any integer in a range of 2 to 10.

14. The electric pulse generator according to claim 2, further comprising:

at least one polarity switching circuit configured to control switching of a polarity of output voltage.

15. The electric pulse generator according to claim 2, further comprising:

at least one pulse control circuit configured to control an output pulse.

16. An electroporator apparatus including the electric pulse generator according to claim 2.

17. The electric pulse generator according to claim 3, wherein the voltage value for switching between the high-voltage mode and the low-voltage mode is a voltage value in a range of 500 to 1000 V.

18. The electric pulse generator according to claim 3, wherein the poring pulse generator includes only one set of a poring pulse generating direct-current power source and a capacitor charging circuit, and a total electric capacity of the n-stage Cockroft-Walton circuit and the second circuit is any value in a range of 500 to 4000 μ

F in the low-voltage mode.

19. The electric pulse generator according to claim 3, wherein the integer n in the n-stage Cockroft-Walton circuit is any integer in a range of 4 to 13, the integer m₁ in the second circuit is any integer in a range of 2 to 6, and the integer m₂ in the second circuit is any integer in a range of 2 to 10.

20. A poring pulse generator, comprising:

an n-stage Cockroft-Walton circuit, where n represents an integer of 2 or more; and

a first circuit that is branched from a branching point of wiring on an output side of the n-stage Cockroft-Walton circuit,

wherein the first circuit includes a switching switch that is turned off in a high-voltage mode and turned on in a low-voltage mode, a voltage value for switching between the high-voltage mode and the low-voltage mode being in a range of 200 to 1400 V, the first circuit includes a second circuit in which m₂ series of m₁ series-connected capacitors are connected in parallel, where m₁ represents an integer of 1 or more, and m₂ represents an integer of 2 or more, the switching switch and the second circuit are connected in series, and first wiring, which is disposed on an output side of the second circuit, is merged with second wiring, which is disposed on the output side of the n-stage Cockroft-Walton circuit, on the output side of the branching point, and a withstand voltage of the n-stage Cockroft-Walton circuit is a value in a range of 1500 to 5000 V in the high-voltage mode.