MICROORGANISM HAVING IMPROVED INTRACELLULAR ENERGY LEVEL AND METHOD FOR PRODUCING L-AMINO ACID USING SAME

• US 20170081633A1
• Filed: 04/14/2015
• Published: 03/23/2017
• Est. Priority Date: 05/23/2014
• Status: Abandoned Application

• Current Assignee: CJ Cheiljedang Corporation (CJ Group)
• Original Assignee: CJ Cheiljedang Corporation (CJ Group)
• Inventors: JANG, Juno, Park, Hye Min, Lee, Kwang Ho, LEE, Keun Cheol, Hong, Hyeong Pyo
• Application Number:
  US15/312,497
• Publication Number:
  US 20170081633A1
• Time in Patent Office: Days
• CPC Class Codes: C07K 14/245 Escherichia (G)
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: C12P 13/04 Alpha- or beta- amino acids other amino acids C12P13/005
• CPC Class Codes: C12P 13/08 Lysine; Diaminopimelic acid; Threonine; Valine
• CPC Class Codes: C12P 13/227 Tryptophan

Abstract

The present application relates to a recombinant microorganism having an improved intracellular energy level and a method for producing L-amino acid using the microorganism.

Claims

1. A microorganism of the genus Escherichia having an increased intracellular ATP level, compared to an unmodified strain, wherein activities of one or more proteins selected from an amino acid sequence of SEQ ID NO:

5, an amino acid sequence of SEQ ID NO;

6, and an amino acid sequence of SEQ ID NO;

7, which constitute an iron uptake system, were inactivated.

2. The microorganism of the genus Escherichia according to claim 1, wherein the activities of all of the proteins having amino acid sequences of SEQ ID NOS:

5, 6, and 7 were inactivated.

3. The microorganism of the genus Escherichia according to claim 1, wherein the microorganism is E. coli.

4. The microorganism of the genus Escherichia according to claim 1, wherein the microorganism of the genus Escherichia has an improved ability to produce L-amino acid, compared to an unmodified strain.

5. The microorganism of the genus Escherichia according to claim 4, wherein the L-amino acid is L-threonine or L-tryptophan.

6. A method for producing L-amino acids, the method comprising:

culturing the microorganism of the genus Escherichia of claim 1 in a media, and

recovering L-amino acids from the culture media or the microorganism.

7. The method of claim 6, wherein the L-amino acid is L-threonine or L-tryptophan.

8. A method for producing L-amino acids, the method comprising:

culturing the microorganism of the genus Escherichia of claim 2 in a media, and

recovering L-amino acids from the culture media or the microorganism.

9. A method for producing L-amino acids, the method comprising:

culturing the microorganism of the genus Escherichia of claim 3 in a media, and

recovering L-amino acids from the culture media or the microorganism.

10. A method for producing L-amino acids, the method comprising:

culturing the microorganism of the genus Escherichia of claim 4 in a media, and

recovering L-amino acids from the culture media or the microorganism.

11. A method for producing L-amino acids, the method comprising:

culturing the microorganism of the genus Escherichia of claim 5 in a media, and

recovering L-amino acids from the culture media or the microorganism.

12. The method of claim 8, wherein the L-amino acid is L-threonine or L-tryptophan.

13. The method of claim 9, wherein the L-amino acid is L-threonine or L-tryptophan.

14. The method of claim 10, wherein the L-amino acid is L-threonine or L-tryptophan.

15. The method of claim 11, wherein the L-amino acid is L-threonine or L-tryptophan.