Microfluidic Analysis System
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Accused Products
Abstract
A microfluidic analysis system (1) performs polymerase chain reaction (PCR) analysis on a bio sample. In a centrifuge (6) the sample is separated into DNA and RNA constituents. The vortex is created by opposing flow of a silicon oil primary carrier fluid effecting circulation by viscous drag. The bio sample exits the centrifuge enveloped in the primary carrier fluid. This is pumped by a flow controller (7) to a thermal stage (9). The thermal stage (9) has a number of microfluidic devices (70) each having thermal zones (71, 72, 73) in which the bio sample is heated or cooled by heat conduction to/from a thermal carrier fluid and the primary carrier fluid. Thus, the carrier fluids envelope the sample, control its flowrate, and control its temperature without need for moving parts at the micro scale.
7 Citations
29 Claims
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1-20. -20. (canceled)
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21. A method for analyzing rare mutated cells from a sample of bodily fluid or tissue using polymerase chain reaction (PCR), the method comprising:
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forming droplets enveloped in an immiscible carrier fluid, wherein the droplets are configured to capture nucleic acid molecules from at least some rare mutated cells; extracting the nucleic acid molecules from the at least some rare mutated cells; amplifying the nucleic acid molecules from the at least some rare mutated cells with PCR; analyzing the nucleic acid molecules from the at least some rare mutated cells, wherein the analysis is configured to target cells that occur about one part in 106. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29)
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Specification