DEVICES AND METHODS USING MODIFIED PAPER ELECTRODES FOR THE DETECTION OF HEMOGLOBIN A1C AND GLUCOSE

• US 20170088875A1
• Filed: 09/26/2016
• Published: 03/30/2017
• Est. Priority Date: 09/25/2015
• Status: Active Grant

• Current Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Original Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Inventors: Henao-Pabon, Gilberto, Li, XiuJun
• Granted Patent: US 10,640,801 B2
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: C07F 5/025 Boronic and borinic acid compounds
• CPC Class Codes: C12Q 1/003 Functionalisation
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 2021/7769 Measurement method of reaction-produced change in sensor
• CPC Class Codes: G01N 33/723 Glycosylated haemoglobin

Abstract

Certain embodiments are directed to sensors that enable the use of optimized biocompatible materials such as pre-anodized paper printed electrode transducer to detect binding of a target agent, wherein the surface is modified or functionalized through zero length cross-linker so that it interacts with or specifically binds a target such as sugars (glucose) or glycated proteins (HgbA1c).

Claims

1. A glycated hemoglobin sensor comprising an anodized support having a functionalized surface coupled to a sugar reactive moiety, wherein the sugar reactive moiety binds glycated hemoglobin.

2. The sensor of claim 1, wherein the support is paper.

3. The sensor of claim 1, wherein the sugar reactive moiety is 3-AminoPhenyl-Boronic Acid (APBA).

4. A diagnostic device comprising the sensor of claim 1.

5. A method of detecting glycated hemoglobin comprising (i) contacting a sensor of claim 1 with a sample to form an exposed sensor, and (ii) contacting the exposed sensor with a glycated hemoglobin detection reagent that produces a detectable signal in the presence of hemoglobin.

6. The method of claim 5, wherein the detection reagent is H₂O₂.

7. The method of claim 5, wherein the detectable signal is an electric signal.

8. The method of claim 7, wherein the signal is generated by direct electron transfer.

9. The method of claim 5, wherein the sample is blood or a blood fraction.

10. A glucose sensor comprising an anodized support having a functionalized surface coupled to a sugar reactive moiety.

11. The sensor of claim 10, wherein the support is paper.

12. The sensor of claim 10, wherein the sugar reactive moiety is glucose oxidase (GOX).

13. A diagnostic device comprising the sensor of claim 10.

14. A method of detecting glucose comprising (i) contacting a sensor of claim 10 with a sample to form an exposed sensor, and (ii) detecting electron transfer in the sensor indicating the presence of glucose.

15. The method of claim 14, wherein the sample is blood or a blood fraction.

16. A diagnostic device having a (i) first anodized support having a functionalized surface coupled to 3-AminoPhenyl-Boronic Acid (APBA), and (ii) second anodized support having a functionalized surface coupled to glucose oxidase (GOX).

17. The device of claim 16, wherein the support surface is functionalized using (1-ethyl-3[3dimethylaminopropyl] carbodiimide hydrochloride (EDC) and N-Hydroxy succimide (NHS).

18. A method of detecting both glucose and HgbA1c simultaneously by contacting a device of claim 16 with a sample and determining HgbA1c binding the first anodized support and glucose binding the second anodized support.

19. The method of claim 18, wherein the sample is blood or a blood fraction.