DEVICES, SYSTEMS AND METHODS FOR LINEARIZATION OF POLYMERS
First Claim
Patent Images
1. A system for sequencing a biopolymer, comprising:
- a nanochannel having a detection region for detecting said biopolymer or portion thereof upon flow of said biopolymer through said detection region; and
a first entropic trapping component and second entropic trapping component disposed in or adjacent to said nanochannel, wherein (i) said first entropic trapping component is operable to linearize said biopolymer upon flow of said biopolymer through said first entropic trapping component and towards a first side of said detection region, and (ii) said second entropic trapping component is operable to linearize said biopolymer upon flow of said biopolymer through said second entropic trapping component and towards a second side of said detection region, which first side is different from said second side.
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Abstract
The present disclosure provides methods and structures for systems which can linearize and capture a nucleic acid molecule (e.g., DNA) for re-measurement of the nucleic acid molecule or other polymer prior to detection of the polymer. The structures may allow for quick exchange between different samples or other reagents.
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Citations
52 Claims
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1. A system for sequencing a biopolymer, comprising:
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a nanochannel having a detection region for detecting said biopolymer or portion thereof upon flow of said biopolymer through said detection region; and a first entropic trapping component and second entropic trapping component disposed in or adjacent to said nanochannel, wherein (i) said first entropic trapping component is operable to linearize said biopolymer upon flow of said biopolymer through said first entropic trapping component and towards a first side of said detection region, and (ii) said second entropic trapping component is operable to linearize said biopolymer upon flow of said biopolymer through said second entropic trapping component and towards a second side of said detection region, which first side is different from said second side. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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20-49. -49. (canceled)
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50. A system for biopolymer concentration, comprising:
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a substrate including a fluid channel and at least one nanochannel in fluid communication with said fluid channel, wherein said fluid channel has a larger cross-section than said at least one nanochannel; and an entropic trapping component comprising a plurality of pillars in said fluid channel, wherein individual pillars of said plurality of pillars are spaced non-uniformly across a cross-section of said fluid channel.
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- 51. A system for biopolymer detection, comprising a substrate including a fluid channel and a plurality of nanochannels in fluid communication with said fluid channel, wherein said fluid channel has a larger cross-section than said plurality of nanochannels, wherein said plurality of nanochannels (i) have different cross-sections or (ii) inlets with non-uniform spacings in said fluid channel.
Specification