METHODS OF GROWING TUMOR INFILTRATING LYMPHOCYTES IN GAS-PERMEABLE CONTAINERS
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Abstract
An embodiment of the invention provides a method of promoting regression of cancer in a mammal comprising obtaining a tumor tissue sample from the mammal; culturing the tumor tissue sample in a first gas permeable container containing cell medium therein; obtaining tumor infiltrating lymphocytes (TIL) from the tumor tissue sample; expanding the number of TIL in a second gas permeable container containing cell medium therein using irradiated allogeneic feeder cells and/or irradiated autologous feeder cells; and administering the expanded number of TIL to the mammal. Methods of obtaining an expanded number of TIL from a mammal for adoptive cell immunotherapy are also provided.
79 Citations
21 Claims
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1. (canceled)
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2. A method of obtaining an expanded number of tumor infiltrating lymphocytes (TIL) from a mammal for adoptive cell immunotherapy comprising:
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(a) obtaining a tumor tissue sample from the mammal; (b) culturing the tumor tissue sample in a first gas permeable container comprising a cell medium and IL-2; (c) obtaining TIL from the tumor tissue sample; and (d) expanding the number of TIL in a second gas permeable container comprising a cell medium, irradiated feeder cells, IL-2, and OKT3. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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Specification