METHOD FOR OPERATING MAGNETIC BODY PARTICLES AND DEVICE FOR OPERATING MAGNETIC BODY PARTICLES

• US 20170152509A1
• Filed: 05/23/2014
• Published: 06/01/2017
• Est. Priority Date: 05/23/2014
• Status: Active Application

• Current Assignee: Shimadzu Corporation
• Original Assignee: Shimadzu Corporation
• Inventors: OHASHI, Tetsuo
• Application Number:
  US15/313,662
• Publication Number:
  US 20170152509A1
• Time in Patent Office: Days
• CPC Class Codes: B01J 20/28004 Sorbent size or size distribution, e.g. particle size
• CPC Class Codes: B01J 20/28009 Magnetic properties
• CPC Class Codes: B03C 1/01 by addition of magnetic adjuvants
• CPC Class Codes: B03C 1/288 disposed at the outer circumference of a recipient
• CPC Class Codes: B03C 1/30 Combinations with other devices, not otherwise provided for
• CPC Class Codes: B03C 2201/18 Magnetic separation whereby the particles are suspended in a liquid
• CPC Class Codes: B03C 2201/26 for use in medical or biological applications
• CPC Class Codes: C07K 1/047 Simultaneous synthesis of different peptide species; Peptide libraries
• CPC Class Codes: C07K 1/22 Affinity chromatography or related techniques based upon selective absorption processes
• CPC Class Codes: C12N 1/066 by physical methods
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
• CPC Class Codes: C12N 15/1013 by using magnetic beads
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: G01N 1/405 by adsorption or absorption
• CPC Class Codes: G01N 2001/4038 electric methods, e.g. electromigration, electrophoresis, ionisation
• CPC Class Codes: G01N 33/54326 Magnetic particles

Abstract

A method for operating magnetic body particles that is for fixing a target substance in a liquid sample to the surface of the magnetic body particles; and a device for operating magnetic body particles that is used for said method. The magnetic body particles are particles to which a target substance can be fixed selectively. In this method, in a state where a liquid sample, the magnetic body particles, and a magnetic solid body that has a larger particle diameter than that of the magnetic body particle are made to coexist in a container, the magnetic body particles are moved together with the magnetic solid body within the liquid sample by operating a magnetic field from outside the container. By this operation, the target substance can be fixed selectively to the surface of the magnetic body particles.

Claims

1-11. -11. (canceled)

12. A method for operating magnetic body particles whereby a target substance in a liquid sample is bound to surfaces of the magnetic body particles, the magnetic body particles being particles capable of selectively binding the target substance, the method comprising:

the magnetic body particles being dispersed in the liquid sample to selectively bind the target substance to surfaces of the magnetic body particles with a magnetic field procedure performed from outside a container in the presence of the liquid sample, the magnetic body particles, and a magnetic solid body having a larger particle diameter than the magnetic body particles in the container to move the magnetic body particles with the magnetic solid body along an inner wall surface of the container in the liquid sample.

13. The method according to claim 12, wherein the target substance which the magnetic body particles are capable of selectively binding is at least one selected from the group consisting of nucleic acids, proteins, sugars, lipids, antibodies, receptors, antigens, ligands, and cells.

14. The method according to claim 12, wherein the liquid sample contains a component capable of lysing a cell.

15. The method according to claim 12, wherein the magnetic solid body has a particle diameter of 100 μ

m or more.

16. The method according to claim 12, wherein the magnetic solid body has a particle diameter that is at least 10 times larger than the particle diameter of the magnetic body particles.

17. The method according to claim 12, wherein the magnetic field procedure moves the magnetic body particles with the magnetic solid body in a reciprocating motion in the liquid sample.

18. The method according to claim 12, wherein the magnetic solid body has a surface with a coating layer for preventing corrosion in the liquid.

19. A method for operating magnetic body particles, comprising:

selectively binding a target substance to surfaces of magnetic body particles according to the method of claim 12; and

contacting the magnetic body particles having the target substance bound thereto to an elution liquid to elute the target substance in the elution liquid.