ELECTROCHEMICAL BIOSENSOR USING DUAL ELECTRODE PAIR

• US 20170166946A1
• Filed: 03/27/2015
• Published: 06/15/2017
• Est. Priority Date: 03/28/2014
• Status: Abandoned Application

• Current Assignee: SK Innovation Company Limited, Ulsan National Institute of Science and Technology
• Original Assignee: SK Innovation Company Limited, Ulsan National Institute of Science and Technology
• Inventors: SHIN, Heung Joo, LIM, Yeong Jin, LEE, Yun-Jeong, DEEPTI, Sharma
• Application Number:
  US15/300,192
• Publication Number:
  US 20170166946A1
• Time in Patent Office: Days
• CPC Class Codes: C12Q 1/003 Functionalisation
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

An electrochemical biosensor using a sensing system includes a working electrode including an active surface modified through a linker; and an auxiliary electrode. The sensor has a high current value compared with an existing sensor and retains excellent stability and sensitivity, and thus can be expected to be easily used for sensing various kinds of biomaterials.

Claims

1. An electrochemical sensor for determining presence or concentration of an analyte in a fluid, comprising:

a substrate; and

a working electrode formed on the substrate, and having an enzyme attached thereon by a linker.

2. The electrochemical sensor of claim 1, wherein the linker is attached on nanoparticles directly formed on the electrode.

3. The electrochemical sensor of claim 2, wherein the nanoparticles are selected from the group consisting of gold, platinum and palladium.

4. The electrochemical sensor of claim 1, further comprising an auxiliary electrode formed to be separated apart from the working electrode in a horizontal direction of the substrate.

5. The electrochemical sensor of claim 1, wherein the working electrode is formed to be separated apart from the substrate in a vertical direction, and has a mesh shape.

6. The electrochemical sensor of claim 5, further comprising:

an auxiliary electrode formed under the working electrode while having a space therebetween.

7. The electrochemical sensor of claim 1, wherein the linker is formed by converting a functional group of a surface-modified base material including diazonium, a diazonium salt or a derivative thereof into an amine group, and then mixing the surface-modified base material with a dialdehyde crosslinking agent.

8. A method of manufacturing an electrochemical sensor, comprising:

forming an electrode on a substrate;

forming a surface-modified base material on the electrode;

reacting the surface-modified base material and a crosslinking agent; and

reacting the reacted crosslinking agent and an enzyme, thereby attaching the enzyme on the electrode.

9. The method of claim 8, wherein the surface-modified base material is diazonium, a diazonium salt or a derivative thereof.

10. The method of claim 9, wherein the surface-modified base material is 4-nitrophenyl diazonium tetrafluoroborate (4-NP).

11. The method of claim 8, wherein the crosslinking agent is dialdehyde.

12. The method of claim 11, wherein the crosslinking agent is glutaraldehyde.

13. The method of claim 8, further comprising:

forming nanoparticles on the electrode,

wherein the surface-modified base material is directly formed on the nanoparticles.