SYSTEMS AND METHODS FOR IMAGE RECONSTRUCTION
First Claim
1. A method for obtaining a high resolution image of one or more objects contained within a sample comprising:
- illuminating the sample with a light source emitting partially coherent light or coherent light, wherein the sample is interposed between the light source and an image sensor with a sample-to-image sensor distance zk;
obtaining a plurality of lower resolution hologram image frames of the objects with the image sensor, wherein the plurality of lower resolution hologram image frames are obtained at different (1) sample-to-image sensor distances zk or (2) different illumination angles θ
k,φ
k;
generating from the plurality of lower resolution hologram image frames a high-resolution initial guess of the objects based on a summation of upsampled holograms in the lower resolution image frames;
iteratively eliminating twin image noise, aliasing signal, and artifacts and retrieving phase information from the high-resolution initial guess, wherein the iterative process comprises;
forward-propagating the high-resolution initial guess from an object plane to an image sensor plane to generate a high-resolution forward-propagated field;
updating an amplitude of the high-resolution forward-propagated field using holograms in the lower resolution hologram image frames;
back-propagating the updated high-resolution forward-propagated field to the object plane;
updating a transmitted field of the object at the object plane in the spatial frequency domain; and
outputting a phase retrieved high resolution image of the one or more objects contained within the sample based on the updated transmitted field of the one or more objects.
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Abstract
A method for obtaining a high resolution image of objects contained within a sample is disclosed that combines pixel super-resolution and phase retrieval techniques into a unified algorithmic framework that enables new holographic image reconstruction methods with significantly improved data efficiency, i.e., using much less number of raw measurements to obtain high-resolution and wide-field reconstructions of the sample. Using the unified algorithmic framework, twin image noise and spatial aliasing signals, along with other digital holographic artifacts, can be interpreted as noise terms modulated by digital phasors, which are all analytical functions of the imaging parameters including e.g., the lateral displacement between the hologram and the sensor array planes (x, y shifts), sample-to-image sensor distance (z), illumination wavelength (λ), and the angle of incidence (θ,φ).
37 Citations
20 Claims
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1. A method for obtaining a high resolution image of one or more objects contained within a sample comprising:
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illuminating the sample with a light source emitting partially coherent light or coherent light, wherein the sample is interposed between the light source and an image sensor with a sample-to-image sensor distance zk; obtaining a plurality of lower resolution hologram image frames of the objects with the image sensor, wherein the plurality of lower resolution hologram image frames are obtained at different (1) sample-to-image sensor distances zk or (2) different illumination angles θ
k,φ
k;generating from the plurality of lower resolution hologram image frames a high-resolution initial guess of the objects based on a summation of upsampled holograms in the lower resolution image frames; iteratively eliminating twin image noise, aliasing signal, and artifacts and retrieving phase information from the high-resolution initial guess, wherein the iterative process comprises; forward-propagating the high-resolution initial guess from an object plane to an image sensor plane to generate a high-resolution forward-propagated field; updating an amplitude of the high-resolution forward-propagated field using holograms in the lower resolution hologram image frames; back-propagating the updated high-resolution forward-propagated field to the object plane; updating a transmitted field of the object at the object plane in the spatial frequency domain; and outputting a phase retrieved high resolution image of the one or more objects contained within the sample based on the updated transmitted field of the one or more objects. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method for obtaining a high resolution image of one or more objects contained within a sample comprising:
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sequentially illuminating the sample at a plurality of different wavelengths with a light source emitting partially coherent light or coherent light, wherein the sample is interposed between the light source and an image sensor with a sample-to-image sensor distance zk; obtaining a plurality of lower resolution hologram image frames of the objects with the image sensor at the plurality of different wavelengths, wherein the plurality of lower resolution hologram image frames are also obtained at different (1) sample-to-image sensor distances zk or (2) different illumination angles θ
k,φ
k;generating from the plurality of lower resolution hologram image frames a high-resolution initial guess of the objects based on a summation of upsampled holograms in the lower resolution image frames; iteratively eliminating twin image noise, aliasing signal, and artifacts and retrieving phase information from the high-resolution initial guess, wherein the iterative process comprises; forward-propagating the high-resolution initial guess from an object plane to an image sensor plane to generate a high-resolution forward-propagated field; updating an amplitude of the high-resolution forward-propagated field using holograms in the lower resolution hologram image frames; back-propagating the updated high-resolution forward-propagated field to the object plane; updating a transmitted field of the object at the object plane in the spatial frequency domain; and outputting a phase retrieved high resolution image of the objects contained within the sample based on the updated transmitted field of the object. - View Dependent Claims (12, 13, 14, 15, 16)
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17. A wavelength scanning pixel super-resolution microscope device for imaging a sample comprising:
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a sample holder configured to hold the sample; a wavelength-tunable light source or multiple different light sources configured to illuminate the sample at a plurality of different wavelengths λ
k along an optical path;a lens or set of lenses disposed along the optical path; an image sensor configured to receive illumination passing through the sample and lens or set of lenses along the optical path, wherein the at least one of the sample holder, lens, set of lenses, or image sensor are moveable along the optical path to introduce incremental defocusing conditions to the microscope device, wherein the image sensor obtains a plurality of images of the sample at the different wavelengths under the incremental defocusing conditions; and at least one processor configured to (1) generate a high-resolution initial guess of the sample image based on the plurality of images of the sample at the different wavelengths under the incremental defocusing conditions, (2) iteratively eliminate artifacts and retrieving phase information from the high-resolution initial guess of the sample image, and (3) output a high resolution, phase retrieved high resolution image of the sample. - View Dependent Claims (18, 19, 20)
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Specification