SELECTIVE OXIDATION OF 5-METHYLCYTOSINE BY TET-FAMILY PROTEINS
First Claim
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1. A method comprising:
- contacting with, or delivering to a somatic cell obtained from a subject, an effective amount of an enzyme or fragment thereof that converts 5-methylcytosine to 5-hydroxymethylcytosine in the somatic cell.
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Abstract
The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.
16 Citations
20 Claims
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1. A method comprising:
contacting with, or delivering to a somatic cell obtained from a subject, an effective amount of an enzyme or fragment thereof that converts 5-methylcytosine to 5-hydroxymethylcytosine in the somatic cell. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. An isolated and purified induced pluripotent stem (iPS) cell, the isolated and purified iPS cell comprising:
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a. induced expression of one or more genes comprising;
Oct-4, Sox2, c-MYC, Klf4, Nanog, Lin-28, or any combination thereof, in the somatic cell; andb. an effective amount of an enzyme or fragment thereof that converts 5-methylcytosine to 5-hydroxymethylcytosine in the iPS cell.
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18. A kit comprising:
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a. an enzyme or fragment thereof that converts 5-methylcytosine to 5-hydroxymethylcytosine; b. one or more nucleic acid sequences that code for a product that induces expression of one or more genes comprising;
Oct-4, Sox2, c-MYC, Klf4, Nanog, Lin-28, or any combination thereof, in a cell; andc. instructions for use. - View Dependent Claims (19, 20)
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Specification