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SELECTIVE OXIDATION OF 5-METHYLCYTOSINE BY TET-FAMILY PROTEINS

  • US 20170176420A1
  • Filed: 02/23/2017
  • Published: 06/22/2017
  • Est. Priority Date: 09/26/2008
  • Status: Abandoned Application
First Claim
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1. A method for detecting 5-methylcytosine in a nucleic acid comprising:

  • a) converting 5-methylcytosine to an acid beyond hydroxymethylation in the nucleic acid using enzymatic modification by a dioxygenase;

    b) bisulfite sequencing the converted nucleic acid; and

    c) detecting the 5-methylcytosine in the nucleic acid at positions where the acid is identified by reading a thymine in the converted nucleic acid where a cytosine is read when the nucleic acid is bisulfite sequenced but not converted.

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