BIOPAPERS AS A SUBSTRATE FOR TISSUE CULTURE

• US 20170183622A1
• Filed: 12/02/2016
• Published: 06/29/2017
• Est. Priority Date: 12/03/2015
• Status: Active Application

• Current Assignee: The Government Of The United States As Represented By The Secretary Of The Navy
• Original Assignee: The Government Of The United States Of America, As Represented By The Secretary Of The Navy
• Inventors: Wu, Peter K., Ringeisen, Bradley R., Pirlo, Russell Kirk, Bischel, Lauren
• Application Number:
  US15/368,049
• Publication Number:
  US 20170183622A1
• Time in Patent Office: Days
• CPC Class Codes: C12M 25/02 Membranes; Filters filters or filtration in general B01D24/00-B01D41/00
• CPC Class Codes: C12N 2533/54 Collagen; Gelatin
• CPC Class Codes: C12N 2537/10 Cross-linking
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: D01D 1/02 Preparation of spinning solutions
• CPC Class Codes: D01D 5/003 the material being a polymer solution or dispersion D01D5/0053 takes precedence
• CPC Class Codes: D01F 1/10 Other agents for modifying properties
• CPC Class Codes: D01F 4/00 Monocomponent artificial filaments or the like of proteins; Manufacture thereof

Abstract

A biocompatible membranes made by electrospinning a polymer, having living cells on both surfaces of the membrane, and/or is free of a non-biodegradable structural component. The membranes may be attached to a frame and span a hole in the frame. A method of: providing a biocompatible membrane made by electrospinning a polymer and depositing living cells both surfaces of the membrane and/or attaching the membrane to a frame and spanning a hole in the frame. Different types of cells are deposited on each surface of the membrane.

Claims

1. An article comprising:

a biocompatible membrane made by electrospinning a polymer; and

a rigid component having a hole;

wherein the membrane is attached to the rigid component and spans the hole.

2. The article of claim 1, wherein the membrane comprises a biodegradable material.

3. The article of claim 2, wherein the membrane is free of a non-biodegradable structural component.

4. The article of claim 2, wherein the membrane comprises a structural component comprising a second biodegradable material that degrades slower that the biodegradable material.

5. The article of claim 1, wherein the membrane comprises non-biodegradable fibers.

6. The article of claim 1, wherein the membrane comprises a non-biodegradable structural component.

7. The article of claim 1, wherein the membrane comprises gelatin.

8. The article of claim 7, wherein the gelatin is crosslinked.

9. The article of claim 1;

wherein rigid components has a plurality of holes; and

wherein the membrane spans each of the plurality of holes.

10. The article of claim 9, wherein the portion of the rigid component between the plurality of holes is the same material as the portion of the rigid component surrounding the plurality of holes.

11. The article of claim 9, wherein the portion of the rigid component between the plurality of holes comprises a biodegradable material.

12. The article of claim 1, wherein the article further comprises:

living cells one or both surfaces of the membrane.

13. The article of claim 12, wherein the surfaces of the membrane comprise different types of cells.

14. The article of claim 13;

wherein human astrocytes are on a first surface of the membrane; and

wherein human brain microvascular endothelial cells are on a second surface of the membrane.

15. The article of claim 13;

wherein human small airway epithelial cells are on a first surface of the membrane; and

wherein human lung microvascular endothelial cells are on a second surface of the membrane.

16. The article of claim 1, wherein the rigid component is a frame comprising a cyclic olefin copolymer.

17. An article comprising:

a biocompatible membrane made by electrospinning a polymer; and

living cells on both surfaces of the membrane;

wherein the surfaces of the membrane comprise different types of cells.

18. The article of claim 17, wherein the membrane comprises a biodegradable material.

19. The article of claim 18, wherein the membrane further comprises non-biodegradable fibers.

20. The article of claim 17, wherein the membrane comprises gelatin.

21. The article of claim 20, wherein the gelatin is crosslinked.

22. The article of claim 17;

wherein human astrocytes are on a first surface of the membrane; and

wherein human brain microvascular endothelial cells are on a second surface of the membrane.

23. The article of claim 17;

wherein human small airway epithelial cells are on a first surface of the membrane; and

wherein human lung microvascular endothelial cells are on a second surface of the membrane.

24. An article comprising:

a biocompatible membrane comprising a polymer that is free of a non-biodegradable structural component; and

a rigid component having a hole;

wherein the membrane is attached to the rigid component and spans the hole.

25. A method comprising:

providing a biocompatible membrane made by electrospinning a polymer; and

attaching the membrane to a rigid component having a hole;

wherein the membrane spans the hole.

26. The method of claim 25, further comprising:

electrospinning the polymer to form the membrane.

27. The method of claim 25, wherein attaching the membrane comprises heat sealing the membrane to the rigid component.

28. The method of claim 25, further comprising:

crosslinking the electrospun polymer by a method that results in a predetermined degradation rate of the membrane.

29. The method of claim 25, further comprising:

depositing living cells one or both surfaces of the membrane.

30. The method of claim 29, wherein different types of cells are deposited on each surface of the membrane.

31. A method comprising:

providing a biocompatible membrane made by electrospinning a polymer; and

depositing living cells both surfaces of the membrane;

wherein different types of cells are deposited on each surface of the membrane.

32. The method of claim 31, further comprising:

electrospinning the polymer to form the membrane.

33. The method of claim 31, further comprising:

crosslinking the electrospun polymer by a method that results in a predetermined degradation rate of the membrane.