RECOMBINANT PROTEINS HAVING FACTOR H ACTIVITY

• US 20170190753A1
• Filed: 12/19/2014
• Published: 07/06/2017
• Est. Priority Date: 12/20/2013
• Status: Active Application

• Current Assignee: Laboratoire francais du Fractionnement et des Biotechnologies
• Original Assignee: Laboratoire francais du Fractionnement et des Biotechnologies
• Inventors: ABACHE, Toufik
• Application Number:
  US15/105,829
• Publication Number:
  US 20170190753A1
• Time in Patent Office: Days
• CPC Class Codes: A01K 67/0275 Genetically modified vertebrates, e.g. transgenic
• CPC Class Codes: C07K 14/472 Complement proteins, e.g. anaphylatoxin, C3a, C5a
• CPC Class Codes: C07K 2319/02 containing a signal sequence
• CPC Class Codes: C07K 2319/21 containing a His-tag

Abstract

The invention relates to a recombinant protein having factor H activity.

Claims

1. A recombinant protein having factor H activity, comprising, from the N-terminus to the C-terminus, a first amino acid sequence comprising at least SCR1 to SCR4 of factor H, and a second amino acid sequence comprising at least SCR19 and SCR20 of factor H, said recombinant protein not being a natural factor H, and provided that if the first sequence consists of SCR1 to SCR4 and the second sequence consists of SCR19 and SCR20, the linker is a synthetic linker.

2. The recombinant protein according to claim 1, the first and/or the second amino acid sequence further comprising one or more other rearranged SCR domains of factor H.

3. The recombinant protein according to claim 1, the first and the second amino acid sequence being linked together by a non-natural linker, in particular a G-A-S-G linker.

4. The recombinant protein according to claim 1, the first or the second amino acid sequence comprising at least SCR12, SCR13 and SCR14 of factor H, or the first and the second amino acid sequence not comprising any of SCR12, SCR13 and SCR14.

5. The recombinant protein according to claim 1, the first sequence comprising SCR1 to SCR4 of factor H, and the second sequence is selected from the group consisting of:

SCR16 to SCR20,

SCR15 to SCR20;

-SCR10 to SCR20; and

SCR8 to SCR20 of factor H.

6. The recombinant protein according to claim 1, the first sequence comprising SCR1 to SCR7 of factor H, and the second sequence comprising SCR16 to SCR20 of factor H, said second sequence being preferably selected from the group consisting of:

SCR15 to SCR20;

SCR14 to SCR20;

SCR11 to SCR20;

SCR10 to SCR20;

SCR9 to SCR20; and

SCR8 to SCR20 of factor H.

7. The recombinant protein according to claim 1, the first sequence comprising SCR1 to SCR8 of factor H, and the second sequence comprising SCR16 to SCR20 of factor H, said second sequence preferably comprising SCR10 to SCR20 of factor H.

8. The recombinant protein according to claim 1, the first sequence comprises SCR1 to SCR4 of factor H and the second sequence comprises SCR16 to SCR20 of factor H, a third sequence comprising SCR7 of factor H being between the first and the second sequence.

9. The recombinant protein according to claim 1, the first amino acid sequence comprising a signal peptide at the N-terminal position, in particular the natural signal peptide of factor H (SEQ ID NO:

24), the signal peptide of a protein different from factor H, or the signal peptide SP-MB7 represented in the sequence SEQ ID NO;

25.

10. The recombinant protein according to claim 1, having the sequence selected from the sequences represented in SEQ ID NO:

26 to 84 and 159.

11. A nucleic acid construct encoding a recombinant protein according to claim 1.

12. The nucleic acid construct according to claim 11, comprising a unique restriction site between the two nucleic acid sequences encoding the first and the second amino acid sequence of the recombinant protein, in particular a NheI site present in the portion encoding a G-A-S-G linker.

13. A vector comprising a nucleic acid construct according to claim 11.

14. A host cell comprising the nucleic acid construct according to claim 11.

15. A nonhuman transgenic animal comprising the nucleic acid construct according to claim 11.

16. A host cell comprising the vector according to claim 13.

17. A nonhuman transgenic animal comprising the vector according to claim 13.