MICROBIAL COMPOSITIONS FOR IMPROVING MILK PRODUCTION IN RUMINANTS

• US 20170196921A1
• Filed: 01/06/2017
• Published: 07/13/2017
• Est. Priority Date: 01/07/2016
• Status: Active Grant

• Current Assignee: Native Microbials Incorporated
• Original Assignee: Ascus Biosciences, Inc.
• Inventors: Embree, Mallory, Picking, Luke, Gogul, Grant, Tarasova, Janna
• Granted Patent: US 10,293,006 B2
• Time in Patent Office: Days
• CPC Class Codes: A23K 10/18 of live microorganisms
• CPC Class Codes: A23K 10/30 from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms obtained by microbiological or biochemical processes, e.g. using yeasts or enzymes, A23K10/10
• CPC Class Codes: A23K 10/37 from waste material from hydrolysates of wood or straw A23K10/32; from molasses A23K10/33
• CPC Class Codes: A23K 20/158 Fatty acids; Fats; Products containing oils or fats
• CPC Class Codes: A23K 20/163 Sugars; Polysaccharides
• CPC Class Codes: A23K 20/28 Silicates, e.g. perlites, zeolites or bentonites
• CPC Class Codes: A23K 40/35 Making capsules specially adapted for ruminants
• CPC Class Codes: A23K 50/10 for ruminants
• CPC Class Codes: A23L 31/10 Yeasts or derivatives thereof
• CPC Class Codes: A23L 33/135 Bacteria or derivatives thereof, e.g. probiotics
• CPC Class Codes: A61K 35/74 Bacteria therapeutic use of a bacterial protein A61K38/00
• CPC Class Codes: A61K 35/741 Probiotics probiotic yeast, e.g. saccharomyces A61K36/06
• CPC Class Codes: A61K 35/742 Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
• CPC Class Codes: A61K 35/748 Cyanobacteria, i.e. blue-green bacteria or blue-green algae, e.g. spirulina algae, microalgae or microphytes A61K36/02
• CPC Class Codes: A61K 36/06 Fungi, e.g. yeasts
• CPC Class Codes: A61K 36/062 Ascomycota
• CPC Class Codes: A61K 36/064 Saccharomycetales, e.g. baker's yeast
• CPC Class Codes: A61K 39/02 Bacterial antigens
• CPC Class Codes: A61K 9/0019 Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner non-active ingredients are additionally classified in A61K47/00
• CPC Class Codes: A61K 9/0053 Mouth and digestive tract, i.e. intraoral and peroral administration rectal administration A61K9/0031
• CPC Class Codes: A61P 15/04 : for inducing labour or abortion; Uterotonics
• CPC Class Codes: A61P 31/04 : Antibacterial agents
• CPC Class Codes: C12N 1/14 : Fungi culture of mushrooms A01G18/00; as new plants A01H15/00; Culture media therefor
• CPC Class Codes: C12N 1/20 : Bacteria; Culture media therefor

Abstract

The disclosure relates to isolated microorganisms—including novel strains of the microorganisms—microbial consortia, and compositions comprising the same. Furthermore, the disclosure teaches methods of utilizing the described microorganisms, microbial consortia, and compositions comprising the same, in methods for modulating the production and yield of milk and milk components in ruminants. In particular aspects, the disclosure provides methods of increasing desirable components of milk in ruminants. Furthermore, the disclosure provides for methods of modulating the rumen microbiome.

Claims

1. A shelf-stable ruminant supplement capable of increasing milk production or improving milk compositional characteristics in a ruminant, comprising:

a) a purified population of Pichia fungi comprising fungi with an ITS nucleic acid sequence that is at least about 97% identical to SEQ ID NO;

32; and

b) a shelf-stable carrier suitable for ruminant administration,

wherein the purified population of Pichia fungi of a) is present in the supplement in an amount effective to increase milk production or improve milk compositional characteristics in a ruminant administered the supplement, as compared to a ruminant not administered the supplement.

2. The shelf-stable ruminant supplement according to claim 1, wherein the purified population of Pichia fungi comprises fungi with an ITS nucleic acid sequence that is at least about 99% identical to SEQ ID NO:

32.

3. The shelf-stable ruminant supplement according to claim 1, wherein the purified population of Pichia fungi comprises fungi with an ITS nucleic acid sequence comprising SEQ ID NO:

32.

4. The shelf-stable ruminant supplement according to claim 1, wherein the purified population of Pichia fungi comprises fungi as deposited at NRRL Y-67249.

5. The shelf-stable ruminant supplement according to claim 1, further comprising:

i. a purified population of bacteria that comprises bacteria with a 16S nucleic acid sequence that is at least about 97% identical to a nucleic acid sequence selected from the group consisting of;

SEQ ID NOs;

1-30 and 2045-2103, and/or

ii. a purified population of fungi that comprises fungi with an ITS nucleic acid sequence that is at least about 97% identical to a nucleic acid sequence selected from the group consisting of;

SEQ ID NOs;

31, 33-60 and 2104-2107.

6. The shelf-stable ruminant supplement according to claim 5, wherein the purified population of bacteria comprises bacteria with a 16S nucleic acid sequence that is at least about 99% identical to a nucleic acid sequence selected from the group consisting of:

SEQ ID NOs;

1-30 and 2045-2103.

7. The shelf-stable ruminant supplement according to claim 5, wherein the purified population of fungi comprises fungi with an ITS nucleic acid sequence that is at least about 99% identical to a nucleic acid sequence selected from the group consisting of:

SEQ ID NOs;

31, 33-60 and 2104-2107.

8. The shelf-stable ruminant supplement according to claim 5, wherein the purified population of bacteria comprises bacteria with a 16S nucleic acid sequence selected from the group consisting of:

SEQ ID NOs;

1-30 and 2045-2103.

9. The shelf-stable ruminant supplement according to claim 5, wherein the purified population of fungi comprises fungi with an ITS nucleic acid sequence selected from the group consisting of:

SEQ ID NOs;

31, 33-60 and 2104-2107.

10. The shelf-stable ruminant supplement according to claim 5, wherein the purified population of bacteria comprises bacteria with a 16S nucleic acid sequence that is at least about 97% identical to SEQ ID NO:

28.

11. The shelf-stable ruminant supplement according to claim 5, wherein the purified population of bacteria comprises bacteria with a 16S nucleic acid sequence that is at least about 99% identical to SEQ ID NO:

28.

12. The shelf-stable ruminant supplement according to claim 5, wherein the purified population of bacteria comprises bacteria with a 16S nucleic acid sequence comprising SEQ ID NO:

28.

13. The shelf-stable ruminant supplement according to claim 5, wherein the purified population of bacteria comprises bacteria as deposited at NRRL B-67248.

14. The shelf-stable ruminant supplement according to claim 5, wherein both a purified population of bacteria i) and a purified population of fungi ii) are present in the supplement.

15. The shelf-stable ruminant supplement according to claim 1, formulated for administration to a cow.

16. The shelf-stable ruminant supplement according to claim 1, wherein the supplement is stable under ambient conditions for at least one week.

17. The shelf-stable ruminant supplement according to claim 1, formulated as an:

encapsulation, tablet, capsule, pill, feed additive, food ingredient, food additive, food preparation, food supplement, consumable solution, consumable spray additive, consumable solid, consumable gel, injection, suppository, bolus, drench, or combinations thereof.

18. The shelf-stable ruminant supplement according to claim 1, wherein the purified population of Pichia fungi is present in the ruminant supplement at a concentration of at least 10² cells.

19. The shelf-stable ruminant supplement according to claim 1, wherein the ruminant administered the supplement exhibits an increase in milk production that leads to a measured increase in milk yield.

20. The shelf-stable ruminant supplement according to claim 1, wherein the ruminant administered the supplement exhibits an increase in milk production and improved milk compositional characteristics that leads to a measured increase in energy-corrected milk.

21. The shelf-stable ruminant supplement according to claim 1, wherein the ruminant administered the supplement exhibits an improved milk compositional characteristic selected from the group consisting of:

an increase in milk fat(s), an increase in milk protein(s), an increase of carbohydrates in milk, an increase of vitamins in milk, an increase of minerals in milk, or combinations thereof.

22. The shelf-stable ruminant supplement according to claim 1, wherein the ruminant administered the supplement exhibits at least a 1% increase in the average production of:

milk fat(s), milk protein(s), energy-corrected milk, or combinations thereof.

23. The shelf-stable ruminant supplement according to claim 1, wherein the ruminant administered the supplement exhibits at least a 10% increase in the average production of:

milk fat(s), milk protein(s), energy-corrected milk, or combinations thereof.

24. The shelf-stable ruminant supplement according to claim 1, wherein the ruminant administered the supplement exhibits at least a 20% increase in the average production of:

milk fat(s), milk protein(s), energy-corrected milk, or combinations thereof.

25. A composition capable of modulating the rumen microbiome of a ruminant, comprising:

a) a purified population of Pichia fungi comprising fungi with an ITS nucleic acid sequence that is at least about 97% identical to SEQ ID NO;

32; and

b) a carrier suitable for ruminant administration,

wherein the purified population of Pichia fungi of a) is present in the composition in an amount effective to cause a shift in the microbiome of the rumen of a ruminant administered the composition.

26. The composition according to claim 25, wherein a population of microbes present in the ruminant'"'"'s rumen before administration of the composition increase in abundance after administration of the composition.

27. The composition according to claim 25, wherein a population of microbes present in the ruminant'"'"'s rumen before administration of the composition decrease in abundance after administration of the composition.

28. The composition according to claim 25, wherein the rumen microbiome of the ruminant administered the composition is shifted to include an increased presence of fiber-degrading genera, volatile fatty acid-producing genera, structural carbohydrate-digesting genera, or combinations thereof.

29. A composition suitable for administration to a ruminant and capable of increasing milk production or improving milk compositional characteristics in a ruminant, comprising:

a) a purified population of fungi as deposited at NRRL Y-67249; and

b) a carrier suitable for ruminant administration,

wherein the purified population of fungi of a) is present in the composition in an amount effective to increase milk production or improve milk compositional characteristics in a ruminant administered the composition, as compared to a ruminant not administered the composition.

30. A composition suitable for administration to a ruminant and capable of increasing milk production or improving milk compositional characteristics in a ruminant, comprising:

a) a purified population of fungi as deposited at NRRL Y-67249;

b) a purified population of bacteria as deposited at NRRL B-67248; and

c) a carrier suitable for ruminant administration,

wherein the purified population of fungi of a) and purified population of bacteria of b) are present in the composition in an amount effective to increase milk production or improve milk compositional characteristics in a ruminant administered the composition, as compared to a ruminant not administered the composition.