PARTICLE SETTLING DEVICES

• US 20170197158A1
• Filed: 07/09/2015
• Published: 07/13/2017
• Est. Priority Date: 07/09/2014
• Status: Abandoned Application

• Current Assignee: Sudhin Biopharma
• Original Assignee: Sudhin Biopharma
• Inventors: KOMPALA, Dhinakar S.
• Application Number:
  US15/324,062
• Publication Number:
  US 20170197158A1
• Time in Patent Office: Days
• CPC Class Codes: B01D 21/0054 Plates in form of a coil
• CPC Class Codes: B01D 21/265 by using a vortex inducer or vortex guide, e.g. coil B01D21/0054 takes precedence
• CPC Class Codes: B04C 5/103 Bodies or members, e.g. bulkheads, guides, in the vortex chamber cores B04C5/107
• CPC Class Codes: C02F 1/385 by centrifuging suspensions centrifuges B04B
• CPC Class Codes: C07K 1/14 Extraction; Separation; Purification
• CPC Class Codes: C12N 1/02 Separating microorganisms from their culture media
• CPC Class Codes: C12N 1/18 Baker's yeast; Brewer's yeast
• CPC Class Codes: C12N 5/00 Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor; plant reproduction by tissue culture techniques A01H4/00
• CPC Class Codes: C12N 5/04 Plant cells or tissues culture media C12N5/0025
• CPC Class Codes: C12N 7/00 Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00
• CPC Class Codes: Y02W 10/37 using solar energy

Abstract

Settling devices for separating millimeter or micron sized particles from a bulk fluid with applications in numerous fields, such as biological (microbial, mammalian, insect, plant, or algal) cell cultures, solid catalyst particle separation from a liquid or gas and waste water treatment.

Claims

1. A particle settling device comprising:

a. a cyclone housing;

b. a spiral vertical plate disposed inside the cyclone housing, the spiral vertical plate joined at one end with a spiral conical surface tapering down to a first opening in the cyclone housing; and

,

c. at least one additional opening in the cyclone housing opposite the first opening.

2. The device of claim 1, wherein the spiral vertical plates comprise at least one material selected from the group consisting of a metal and a plastic.

3. The device of claim 1, wherein the spiral vertical plates comprise stainless steel.

4. The device of claim 1, composed entirely of stainless steel.

5. The device of claim 1, composed entirely of plastic.

6. The device of any one of claims 2-4, wherein the spiral vertical plate and the spiral conical surface are metals joined by welding.

7. The device of any one of claims 1-6, wherein the spiral vertical plate is arranged in a substantially vertical position within the cyclone housing and an angle of inclination for the conical spiral surface is between about 30 degrees and about 60 degrees from vertical.

8. The device of claim 7, wherein the angle of inclination for the conical spiral surface is about 30 degrees from vertical.

9. The device of claim 7, wherein the angle of inclination for the conical spiral surface is about 60 degrees from vertical.

10. The device of claim 1, wherein at least one surface of the cyclone housing, the spiral vertical plate or the spiral conical surface is coated with a non-sticky plastic or silicone.

11. The device of claim 1, wherein a width of a channel formed between adjacent surfaces of the spiral vertical plate is between about 1 mm and about 50 mm.

12. The device of claim 1, wherein a number of spirals forming adjacent surfaces of the spiral vertical plate is between about 3 and about 30.

13. The device of claim 1, wherein the first opening is configured as an outlet in liquid communication with the outside and the inside of the cyclone housing substantially opposite the spiral vertical plate.

14. The device of claim 13, further comprising a closure over at least a portion of the cyclone housing at an end of the cyclone housing adjacent the spiral vertical plate and opposite the first opening.

15. The device of claim 14, wherein the at least one additional opening in the cyclone housing is configured to open from a side of the cyclone housing tangential to the spiral vertical plate, in liquid communication with the outside and the inside of the cyclone housing.

16. The device of claim 15, further comprising a liquid harvest outlet formed in the closure, in liquid communication with the outside and the inside of the cyclone housing.

17. The device of claim 14, wherein the at least one additional opening in the cyclone housing is configured to open into the closure, in liquid communication with the outside and the inside of the cyclone housing.

18. The device of claim 17, further comprising a liquid harvest outlet formed in a side of the cyclone housing tangential to the spiral vertical plate, in liquid communication with the outside and the inside of the cyclone housing.

19. The device of claim 14, wherein the first opening is in liquid communication with the outside and the inside of the cyclone housing and is configured to communicate liquid both into and out of the cyclone housing.

61. The device of claim 1, wherein a width of a channel formed between adjacent surfaces of the at least two conical plates is between about 1 mm and about 50 mm.

62. The device of claim 1, wherein a number of conical plates in the stack of at least two conical plates is between about 3 and about 30 conical plates.

20. A particle settling device comprising:

a. a cyclone housing;

b. a spiral vertical plate disposed inside the cyclone housing opposite a first opening in the cyclone housing, and,

c. at least one additional opening in the cyclone housing opposite the first opening.

21. The device of claim 20, further comprising a spiral conical surface joined to one end of the spiral vertical plate, the spiral conical surface tapering down to the first opening in the cyclone housing;

22. The device of claim 20, wherein the spiral vertical plates comprise at least one material selected from the group consisting of a metal and a plastic.

23. The device of claim 20, wherein the spiral vertical plates comprise stainless steel.

24. The device of claim 20, composed entirely of stainless steel.

25. The device of claim 20, composed entirely of plastic.

26. The device of any one of claims 20-25, wherein the spiral vertical plate is arranged in a substantially vertical position within the cyclone housing.

27. The device of claim 20, wherein at least one surface of the cyclone housing, the spiral vertical plate or the spiral conical surface is coated with a non-sticky plastic or silicone.

28. The device of claim 20, wherein a width of a channel formed between adjacent surfaces of the spiral vertical plate is between about 1 mm and about 50 mm.

29. The device of claim 20, wherein a number of spirals forming adjacent surfaces of the spiral vertical plate is between about 3 and about 30.

30. The device of claim 20, wherein the first opening is configured as an outlet in liquid communication with the outside and the inside of the cyclone housing substantially opposite the spiral vertical plate.

31. The device of claim 30, further comprising a closure over at least a portion of the cyclone housing at an end of the cyclone housing adjacent the spiral vertical plate and opposite the first opening.

32. The device of claim 20, wherein the at least one additional opening in the cyclone housing is configured to open from a side of the cyclone housing tangential to the spiral vertical plate, in liquid communication with the outside and the inside of the cyclone housing.

33. The device of claim 32, further comprising a liquid harvest outlet formed in the closure, in liquid communication with the outside and the inside of the cyclone housing.

34. The device of claim 32, wherein the at least one additional opening in the cyclone housing is configured to open into the closure, in liquid communication with the outside and the inside of the cyclone housing.

35. The device of claim 32, further comprising a liquid harvest outlet formed in a side of the cyclone housing tangential to the spiral vertical plate, in liquid communication with the outside and the inside of the cyclone housing.

36. The device of claim 32, wherein the first opening is in liquid communication with the outside and the inside of the cyclone housing and is configured to communicate liquid both into and out of the cyclone housing.

37. A particle settling device comprising:

a. a cyclone housing;

b. a spiral vertical plate disposed inside the cyclone housing, wherein a height of the spiral vertical plate decreases from the exterior to the interior of the spiral;

c. a spiral conical surface joined at one end to the spiral vertical plate tapering down to a first opening in the cyclone housing; and

,

d. at least one additional opening in the cyclone housing opposite the first opening.

38. The device of claim 37, composed entirely of a plastic.

39. The device of claim 37, wherein the spiral vertical plate is arranged in a substantially vertical position within the cyclone housing and an angle of inclination for the conical spiral surface is between about 30 degrees and about 60 degrees from vertical.

40. The device of claim 39, wherein the angle of inclination for the conical spiral surface is about 30 degrees from vertical.

41. The device of claim 39, wherein the angle of inclination for the conical spiral surface is about 60 degrees from vertical.

42. The device of claim 37, wherein at least one surface of the cyclone housing, the spiral vertical plate or the spiral conical surface is coated with a non-sticky plastic or silicone.

43. The device of claim 37, wherein a width of a channel formed between adjacent surfaces of the spiral vertical plate is between about 1 mm and about 50 mm.

44. The device of claim 37, wherein a number of spirals forming adjacent surfaces of the spiral vertical plate is between about 3 and about 30.

45. The device of claim 37, wherein the first opening is configured as an outlet in liquid communication with the outside and the inside of the cyclone housing substantially opposite the spiral vertical plate.

46. The device of claim 45, further comprising a closure over at least a portion of the cyclone housing at an end of the cyclone housing adjacent the spiral vertical plate and opposite the first opening.

47. The device of claim 37, wherein the at least one additional opening in the cyclone housing is configured to open from a side of the cyclone housing tangential to the spiral vertical plate, in liquid communication with the outside and the inside of the cyclone housing.

48. The device of claim 47, further comprising a liquid harvest outlet formed in the closure, in liquid communication with the outside and the inside of the cyclone housing.

49. The device of claim 48, further comprising a liquid harvest outlet formed in a side of the cyclone housing tangential to the spiral vertical plate, in liquid communication with the outside and the inside of the cyclone housing.

50. The device of claim 37, wherein the first opening is in liquid communication with the outside and the inside of the cyclone housing and is configured to communicate liquid both into and out of the cyclone housing.

51. A particle settling device comprising:

a. a cyclone housing;

b. at least two conical plates disposed inside the cyclone housing, the at least two conical plates stacked one above the other, each of the at least two conical plates comprising at least three vertical supports that project from a surface of each conical plate to hold each conical plate at a substantially constant distance from a next conical plate in the stack of at least two conical plates, each of the conical plates comprising an opening sufficient to allow liquid to pass through the openings in the conical plates, the stack of at least two conical plates tapering down to a first opening in the cyclone housing; and

,

c. at least one additional opening in the cyclone housing opposite the first opening.

52. The device of claim 51, wherein the at least two conical plates comprise at least one material selected from the group consisting of a metal and a plastic.

53. The device of claim 51, wherein the at least two conical plates comprise stainless steel.

54. The device of claim 51, composed entirely of stainless steel.

55. The device of claim 51, composed entirely of plastic.

56. The device of any one of claims 52-54, wherein the spiral vertical plate and the spiral conical surface are metals joined by welding.

57. The device of any one of claims 51-56, wherein the stack of at least two conical plates is arranged in a substantially vertical position within the cyclone housing and an angle of inclination for a surface of each of the at least two conical plates is between about 30 degrees and about 60 degrees from vertical.

58. The device of claim 57, wherein the angle of inclination for the surface is about 30 degrees from vertical.

59. The device of claim 57, wherein the angle of inclination for the surface is about 60 degrees from vertical.

60. The device of claim 51, wherein at least one surface of the cyclone housing, and the at least two conical plates is coated with a non-sticky plastic or silicone.

63. The device of claim 51, wherein the first opening is configured as an outlet in liquid communication with the outside and the inside of the cyclone housing.

64. The device of claim 51, further comprising a closure over at least a portion of the cyclone housing at an end of the cyclone housing opposite the first opening.

65. The device of claim 64, further comprising a liquid harvest outlet formed in the closure, in liquid communication with the outside and the inside of the cyclone housing.

66. The device of claim 65, further comprising at least one additional opening in the cyclone housing configured to open into the closure, in liquid communication with the outside and the inside of the cyclone housing.

67. The device of claim 51, further comprising at least one additional opening in the cyclone housing configured to open from a side of the cyclone housing tangential to the at least two conical plates, in liquid communication with the outside and the inside of the cyclone housing.

68. The device of claim 51, further comprising a liquid harvest outlet formed in a side of the cyclone housing tangential to the at least two conical plates, in liquid communication with the outside and the inside of the cyclone housing.

69. The device of claim 51, wherein the first opening is in liquid communication with the outside and the inside of the cyclone housing and is configured to communicate liquid both into and out of the cyclone housing.

70. A method of settling particles in a liquid suspension, comprising:

a. introducing a liquid suspension into a particle settling device comprising;

i. a cyclone housing;

ii. a spiral vertical plate disposed inside the cyclone housing, the spiral vertical plate joined at one end with a spiral conical surface tapering down to a first opening in the cyclone housing; and

,

iii. at least one additional opening in the cyclone housing opposite the first opening configured to receive the liquid suspension,

b. collecting particles from the first opening; and

,

c. collecting a liquid from the settling device.

71. The method of claim 70, wherein the particle settling device further comprises a closure over at least a portion of the cyclone housing at an end of the cyclone housing adjacent the spiral vertical plate and opposite the first opening, and liquid is collected from the particle settling device from at least one opening formed in the closure.

72. The method of claim 70, wherein the at least one additional opening in the cyclone housing is configured to open from a side of the cyclone housing tangential to the spiral vertical plate, in liquid communication with the outside and the inside of the cyclone housing.

73. The method of claim 70, wherein the liquid suspension comprises at least one of a recombinant cell suspension, an alcoholic fermentation, a suspension of solid catalyst particles, a municipal waste water, industrial waste water.

74. The method of claim 70, wherein the liquid suspension comprises at least one of mammalian cells, bacterial cells, yeast cells, plant cells, insect cells.

75. The method of claim 70, wherein the liquid suspension comprises at least one of biodiesel-producing algae cells, mammalian and/or murine hybridoma cells, and yeast in beer.

76. The method of claim 70, wherein the liquid suspension comprises recombinant microbial cells selected from Pichia pastoris, Saccharomyces cerevisiae, Kluyveromyces lactis, Aspergillus niger, Escherichia coli, and Bacillus subtilis.

77. The method of claim 70, wherein the step of introducing a liquid suspension comprises directing a liquid suspension from a plastic disposable bioreactor bag into the particle settling device.

78. The method of claim 70, wherein the liquid collected comprises at least one of biological molecules, organic or inorganic compounds, chemical reactants, and chemical reaction products.

79. The method of claim 70, wherein the liquid collected comprises at least one of hydrocarbons, polypeptides, proteins, alcohols, fatty acids, hormones, carbohydrates, antibodies, isoprenoids, biodiesel, and beer.

80. The method of claim 70, wherein the liquid collected comprises at least one of insulin or its analogs, monoclonal antibodies, growth factors, sub-unit vaccines, viruses, virus-like particles, colony stimulating factors and erythropoietin (EPO).