NANOPORE BASED SEQUENCER

• US 20170204066A1
• Filed: 01/27/2017
• Published: 07/20/2017
• Est. Priority Date: 10/06/2008
• Status: Active Grant

• Current Assignee: Arizona Board of Regents (University of Arizona)
• Original Assignee: Arizona Board of Regents (University of Arizona)
• Inventors: ZHANG, Peiming, LINDSAY, Stuart
• Granted Patent: US 10,442,771 B2
• Time in Patent Office: Days
• CPC Class Codes: C07D 235/24 with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached in position 2
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: C12Q 2565/631 being a biochannel or pore
• CPC Class Codes: G01N 27/447 using electrophoresis
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027
• CPC Class Codes: G01N 33/48721 Investigating individual macromolecules, e.g. by translocation through nanopores Coulter counters in general G01N15/12; fabrication methods for nanoscale apertures B81B1/00; sequencing of nucleic acids C12Q1/68

Abstract

The present invention is directed to systems, devices and methods for identifying biopolymers, such as strands of DNA, as they pass through a constriction such as a carbon nanotube nanopore. More particularly, the invention is directed to such systems, devices and methods in which a newly translocated portion of the biopolymer forms a temporary electrical circuit between the nanotube nanopore and a second electrode, which may also be a nanotube. Further, the invention is directed to such systems, devices and methods in which the constriction is provided with a functionalized unit which, together with a newly translocated portion of the biopolymer, forms a temporary electrical circuit that can be used to characterize that portion of the biopolymer.

Claims

1-17. -17. (canceled)

18. A compound for trapping and reading an analyte in a tunnel junction, the compound having a structure comprising a five membered aromatic ring fused either with a conductive ring moiety, or a derivative of the conductive ring moiety, thereby enhancing the conductivity of the compound.

19. The compound according to claim 18, wherein the compound is 5(6)-mercapto-1H-benzo[d]imidazole-2-carboxamide.

20. A composition comprising the compound of claim 18.

21. A compound of a reader molecule for use in a recognition tunneling apparatus, the reader molecule comprising a fusion of an aromatic ring with a heterocycle, wherein the reader molecule forms complexes with biochemical molecules through non-covalent interactions, the interactions comprising at least one of hydrogen bonding and stacking interaction, wherein as a result of the formed complexes, tunneling current signals are generated in the recognition tunneling apparatus.

22. The compound according to claim 21, wherein the compound is 5(6)-mercapto-1H-benzo[d]imidazole-2-carboxamide.

23. A composition comprising the molecule of claim 21.

24. A compound of the formula C₈H₇N₃OS, having a structure given by:

25. A composition comprising the compound of claim 24.