METHOD FOR DETECTING AND QUANTIFYING FIBROSIS
First Claim
1. A method for detecting and quantifying fibrosis, the method comprising the steps of:
- positioning a fixed non-colored tissue sample on a support;
performing an infrared acquisition by sweeping points of said sample;
producing an infrared spectrum, wherein each acquired point is converted into at least one spectral image;
digital processing of the spectral image is carried out by discrimination between at least physiological collagen and pathological collagen from fibrosis by assigning to each point a spectral class defined by an algorithm classifying the spectra according to spectral similarities;
assigning a condition to each class by statistical comparison with a numerical model of spectra;
quantifying a relative surface area occupied by the points of each condition; and
recording a result.
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Accused Products
Abstract
A method for detecting and quantifying fibrosis, which involves at least; positioning a non-coloured fixed tissue sample on a support; carrying out infrared acquisition, by scanning, of points of the sample; converting the infrared spectrum produced by each acquired point into at least one spectral image; carrying out a digital processing operation on the spectral image by distinguishing between at least physiological collagen and pathological collagen from fibrosis, attributing, to each point, a spectral class defined by an algorithm classifying the spectra according to the spectral similarities % thereof, then attributing a status to each class by statistically comparing with a digital model of spectra; quantifying the relative surface area occupied by the points of each status; and recording the result.
5 Citations
9 Claims
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1. A method for detecting and quantifying fibrosis, the method comprising the steps of:
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positioning a fixed non-colored tissue sample on a support; performing an infrared acquisition by sweeping points of said sample; producing an infrared spectrum, wherein each acquired point is converted into at least one spectral image; digital processing of the spectral image is carried out by discrimination between at least physiological collagen and pathological collagen from fibrosis by assigning to each point a spectral class defined by an algorithm classifying the spectra according to spectral similarities; assigning a condition to each class by statistical comparison with a numerical model of spectra; quantifying a relative surface area occupied by the points of each condition; and recording a result. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A device for detecting and quantifying fibrosis, said device comprising:
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means for receiving a support of a tissue sample; means for acquisition by Fourier transform infrared spectroscopy, positioned facing said receiving means, said means acquisition converting the infrared spectrum produced by each acquired point into at least one spectral image; means for digital processing of said spectral image by discrimination between at least physiological collagen and pathological collagen by assigning to each point a spectral class by statistical comparison with a numerical model of spectra with which said processing means are provided, said processing means quantifying the points having a similar spectral class; and means for digitally recording the result of said processing means. - View Dependent Claims (9)
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Specification