INOCULANTS AND METHODS FOR USE THEREOF

• US 20170215358A1
• Filed: 06/19/2015
• Published: 08/03/2017
• Est. Priority Date: 06/20/2014
• Status: Active Grant

• Current Assignee: Flinders University of South Australia
• Original Assignee: Flinders University of South Australia
• Inventors: FRANCO, Christopher Milton Mathew, LE, Hoang Xuyen, BALLARD, Ross Alexander
• Granted Patent: US 10,462,990 B2
• Time in Patent Office: Days
• CPC Class Codes: A01H 3/00 Processes for modifying phenotypes , e.g. symbiosis with bacteria A01H4/00 takes precedence
• CPC Class Codes: A01N 63/20 Bacteria; Substances produced thereby or obtained therefrom
• CPC Class Codes: A01N 63/28 Streptomyces
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12N 15/8261 with agronomic (input) traits, e.g. crop yield
• CPC Class Codes: C12N 15/8279 for biotic stress resistance, pathogen resistance, disease resistance
• CPC Class Codes: C12R 2001/465 Streptomyces

Abstract

The present invention relates to methods for enhancing at least one growth parameter of a leguminous plant via co-inoculation of a leguminous plant with at least one rhizobial microorganism together with at least one actinobacterial microorganism. In further aspects, the present invention also relates to leguminous plants co-inoculated with at least one rhizobial microorganism together with at least one actinobacterial microorganism, as well as specific actinobacterial strains and inoculant compositions which are useful in accordance with the present invention.

Claims

1. A method for enhancing at least one growth parameter of a leguminous plant, the method comprising co-inoculating the leguminous plant with:

at least one rhizobial microorganism; and

at least one actinobacterial microorganism;

wherein the co-inoculated leguminous plant has at least one enhanced growth parameter relative to a leguminous plant of the same taxon that has not been co-inoculated.

3. The method of claim 1 wherein the actinobacterial microorganism is from a genus selected from Streptomyces, Microbispora or Micromonospora.

6. The method of claim 1 wherein the actinobacterial microorganism comprises a 16S rRNA gene nucleotide sequence which is at least 90% identical to one or more of:

SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, SEQ ID NO;

7, SEQ ID NO;

8, SEQ ID NO;

9 or SEQ ID NO;

10.

13. The method of claim 1 wherein the growth parameter is a number and/or mass of nodules of the leguminous plant.

14. The method of claim 1 wherein the growth parameter is a number and/or mass of seed pods and/or seed produced by the leguminous plant.

15. The method of claim 1 wherein the growth parameter is a concentration and/or amount of a nutrient in the leguminous plant.

16. The method of claim 15 wherein the nutrient is selected from:

Boron, Calcium, Copper, Magnesium, Manganese, Phosphorous, Sodium, Sulphur, Nitrogen and/or Zinc.

18. The method of claim 1 wherein the growth parameter is a germination rate of a leguminous plant.

19. The method of claim 1 wherein the rhizobial microorganism is a Sinorhizobium sp.

20. The method of claim 1 wherein the rhizobial microorganism is a Rhizobium sp.

21. The method of claim 1 wherein the rhizobial microorganism is a Bradyrhizobium sp.

22. The method of claim 1 wherein the leguminous plant is selected from the group of:

a-Medicago sp., a Trifolium sp., a Pisum sp., or a Glycine sp.

23. The method of claim 1 wherein the leguminous plant is exposed to a pathogen and, when exposed to the pathogen, the co-inoculated leguminous plant has at least one enhanced growth parameter relative to a leguminous plant of the same taxon that has not been co-inoculated.

24. The method of claim 23 wherein the pathogen is a root pathogen.

25. The method of claim 23 wherein the pathogen is a fungal pathogen.

26. The method of claim 25 wherein the pathogen is a Rhizoctonia sp.

2. (canceled)

4-5. -5. (canceled)

7-12. -12 (canceled)

17. (canceled)

27. (canceled)

28. A leguminous plant, leguminous plant part or leguminous plant reproductive material, co-inoculated with at least one rhizobial microorganism and at least one actinobacterial microorganism.

30. The leguminous plant, leguminous plant part or leguminous plant reproductive material of claim 28 wherein the actinobacterial microorganism is from a genus selected from Streptomyces, Microbispora or Micromonospora.

39. The leguminous plant, leguminous plant part or leguminous plant reproductive material of claim 28 wherein the rhizobial microorganism is a Rhizobium sp.

29. (canceled)

31-38. -38. (canceled)

40-62. -62. (canceled)

63. An isolated actinobacterial microorganism wherein the actinobacterial microorganism is Streptomyces sp. LuP30 as deposited under NMI accession number V13/030101, Streptomyces sp. LuP47B as deposited under NMI accession number V13/030100, Streptomyces sp. EN23 as deposited under as AGAL Deposit No. NM03/35605, or Streptomyces sp. EN27 as deposited as AGAL Deposit No. NM03/35606.