GENETIC ENGINEERING OF NON-HUMAN ANIMALS FOR THE PRODUCTION OF CHIMERIC ANTIBODIES
First Claim
1. A mammalian cell whose genome comprises a transgene comprising a gene segment encoding an immunoglobulin heavy chain variable (V) polypeptide, a gene segment encoding an immunoglobulin heavy chain diversity (D) polypeptide, a gene segment encoding an immunoglobulin heavy chain joining (J) polypeptide, and an immunoglobulin heavy chain non-coding element for the V gene segment, wherein the V polypeptide and the J polypeptide are from a first species, wherein the non-coding element is from a second species, and wherein the V gene segment, D gene segment, and J gene segment replace an immunoglobulin heavy chain V coding sequence, an immunoglobulin heavy chain D coding sequence, and an immunoglobulin heavy chain J coding sequence, respectively, of the second species.
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Abstract
The invention provides non-human cells and mammals having a genome encoding chimeric antibodies and methods of producing transgenic cells and mammals. Certain aspects of the invention include chimeric antibodies, humanized antibodies, pharmaceutical compositions and kits. Certain aspects of the invention also relate to diagnostic and treatment methods using the antibodies of the invention.
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Citations
20 Claims
- 1. A mammalian cell whose genome comprises a transgene comprising a gene segment encoding an immunoglobulin heavy chain variable (V) polypeptide, a gene segment encoding an immunoglobulin heavy chain diversity (D) polypeptide, a gene segment encoding an immunoglobulin heavy chain joining (J) polypeptide, and an immunoglobulin heavy chain non-coding element for the V gene segment, wherein the V polypeptide and the J polypeptide are from a first species, wherein the non-coding element is from a second species, and wherein the V gene segment, D gene segment, and J gene segment replace an immunoglobulin heavy chain V coding sequence, an immunoglobulin heavy chain D coding sequence, and an immunoglobulin heavy chain J coding sequence, respectively, of the second species.
Specification