LINKER ELEMENT AND METHOD OF USING SAME TO CONSTRUCT SEQUENCING LIBRARY
First Claim
1. A linker element consisting of a linker A and a linker B, wherein the linker A is generated from the complementary pairing of a long strand of nucleic acid and a short strand of nucleic acid, wherein the long strand has a phosphate modification at the 5′
- end and the short strand has a blocking modification at the 3′
end, and has an enzyme active site in the short strand; and
the linker B is a single-stranded nucleic acid, and the 3′
end thereof can be complementary to the 5′
end of the long strand of the linker A but the rest part cannot be complementary to the linker A.
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Accused Products
Abstract
Provided is a linker element and a method of using the linker element to construct a sequencing library, wherein the linker element consists of a linker A and a linker B, the linker A is obtained through the complementary pairing of a long nucleic acid strand and a short nucleic acid strand, the 5′ end of the long strand has a phosphoric acid modification, and the 3′ end of the short strand has an enclosed modification, with enzyme sites in the short strand; and the linker B is a nucleic acid single strand, and the 3′ end thereof can be in a complementary pairing with the 5′ end of the long strand of the linker A. Using the linker element of the present invention for constructing a sequencing library ensures the linking directionality of the linkers while solving the problems of fragment interlinking, linker self-linking and low linking efficiency, and reducing the purification reaction between steps, shortening the linking time and reducing costs.
1 Citation
26 Claims
-
1. A linker element consisting of a linker A and a linker B, wherein the linker A is generated from the complementary pairing of a long strand of nucleic acid and a short strand of nucleic acid, wherein the long strand has a phosphate modification at the 5′
- end and the short strand has a blocking modification at the 3′
end, and has an enzyme active site in the short strand; and
the linker B is a single-stranded nucleic acid, and the 3′
end thereof can be complementary to the 5′
end of the long strand of the linker A but the rest part cannot be complementary to the linker A. - View Dependent Claims (2, 7, 8, 10, 11, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
- end and the short strand has a blocking modification at the 3′
-
3-6. -6. (canceled)
-
9. (canceled)
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12-15. -15. (canceled)
Specification