DEVICES AND METHODS FOR TARGET ANALYTE DETECTION IN LIQUID SAMPLES
First Claim
1. A device for detecting a target analyte in a liquid sample, the device comprising:
- a housing that includes;
an inlet for receiving a liquid sample;
an outlet for removing a volume of the liquid sample from the device;
a filter associated with the outlet and being sized and dimensioned to retain a target analyte on a surface thereof; and
a flow system comprising at least one channel that is in communication with the inlet and the outlet, at least a portion of the at least one channel being located substantially adjacent the surface of the filter and shaped and dimensioned to reduce the amount of unreacted fluorescent probe available to create background fluorescence during detection of the target analyte.
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Abstract
One aspect of the present disclosure relates to a device for detecting a target analyte in a liquid sample. The device can comprise a housing. The housing can include an inlet for receiving a liquid sample, an outlet for removing a volume of the liquid sample from the device, a filter associated with the outlet and being sized and dimensioned to retain a target analyte on a surface thereof, and a flow system comprising at least one channel that is in communication with the inlet and the outlet. At least a portion of the at least one channel can be located substantially adjacent the surface of the filter and be shaped and dimensioned to reduce the amount of unreacted fluorescent probe available to create the background interference during detection of the target analyte.
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Citations
20 Claims
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1. A device for detecting a target analyte in a liquid sample, the device comprising:
a housing that includes; an inlet for receiving a liquid sample; an outlet for removing a volume of the liquid sample from the device; a filter associated with the outlet and being sized and dimensioned to retain a target analyte on a surface thereof; and a flow system comprising at least one channel that is in communication with the inlet and the outlet, at least a portion of the at least one channel being located substantially adjacent the surface of the filter and shaped and dimensioned to reduce the amount of unreacted fluorescent probe available to create background fluorescence during detection of the target analyte. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A method for detecting a target analyte in a liquid sample, the method comprising the steps of:
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introducing, through an inlet of a device, a liquid sample either before, during, or after introduction of a detection reagent through the inlet, the device including a flow system comprising at least one channel that is in communication with the inlet and an outlet, at least a portion of the at least one channel being located substantially adjacent the surface of the filter and shaped and dimensioned to reduce the amount of unreacted fluorescent probe available to create the background fluorescence; removing, from an outlet of the device, a volume of the liquid sample to cause a target analyte, if present, to be retained on a surface of a filter associated with the outlet; exposing the target analyte to a detection reagent specific to the target analyte; and determining the presence of the target analyte in the liquid sample. - View Dependent Claims (13, 14, 15, 16)
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17. A method for detecting a target pathogen, or biomolecule associated therewith, in a liquid sample, the method comprising the steps of:
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introducing, through an inlet of a device, a liquid sample either before, during, or after introduction of a fluorescent probe specific to the target pathogen, or biomolecule associated therewith, through the inlet; removing, from an outlet of the device, a volume of the liquid sample to cause a target pathogen, or biomolecule associated therewith, if present, to be retained on a surface of a filter associated with the outlet; actuating a release mechanism of the device to cause release of the retained target pathogen, or biomolecule associated therewith, off of the surface of the filter; and determining, by fluoroscopy, the presence of the target pathogen, or biomolecule associated therewith, in the liquid sample; whereby release of the target pathogen, or biomolecule associated therewith, reduces background fluorescence associated with the fluorescent probe during the determining step. - View Dependent Claims (18, 19, 20)
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Specification