ANTI-PD-1 MONOCLONAL ANTIBODY AND OBTAINING METHOD THEREFOR
First Claim
1. An anti-PD-1 monoclonal antibody comprises light chains and heavy chains, wherein the light chain contains three complementarity determining regions (CDRs) which are named as LCDR1, LCDR2 and LCDR3;
- the LCDR1 comprises one of peptides whose amino acid sequences are shown as of SEQ ID NO. 18, SEQ ID NO. 19, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 23 or SEQ ID NO. 24;
the LCDR2 comprises one of the peptide whose amino acid sequences are shown as SEQ ID NO. 25, SEQ ID NO. 26, SEQ ID NO. 27, SEQ ID NO. 28, SEQ ID NO. 29 or SEQ ID NO. 30;
the LCDR3 comprises one of the peptides whose amino acid sequences shown as SEQ ID NO. 31, SEQ ID NO. 32, SEQ ID NO. 33, SEQ ID NO. 34, SEQ ID NO. 35, SEQ ID NO. 36 or SEQ ID NO. 37.
1 Assignment
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Accused Products
Abstract
The invention provides human monoclonal antibodies that specifically bind to PD-1 with high affinity. The anti-PD-1 monoclonal antibodies were screened from a synthetic antibody library, and affinity maturation was performed. The synthetic antibody libraries used to select for the high affinity anti-PD-1 monoclonal antibodies were made by replacing the light chain CDR1, CDR2 and CDR3 and heavy chain CDR1, CDR 2 and CDR 3 of phage libraries from the preliminary screening, and the high affinity anti-PD-1 monoclonal antibodies were selected. The human anti-PD-1 monoclonal antibodies have high affinity and inhibit the binding of PD-1 to its ligand PD-L1. The antibodies can be used for treating tumor, inflammation and autoimmune diseases.
13 Citations
12 Claims
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1. An anti-PD-1 monoclonal antibody comprises light chains and heavy chains, wherein the light chain contains three complementarity determining regions (CDRs) which are named as LCDR1, LCDR2 and LCDR3;
- the LCDR1 comprises one of peptides whose amino acid sequences are shown as of SEQ ID NO. 18, SEQ ID NO. 19, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 23 or SEQ ID NO. 24;
the LCDR2 comprises one of the peptide whose amino acid sequences are shown as SEQ ID NO. 25, SEQ ID NO. 26, SEQ ID NO. 27, SEQ ID NO. 28, SEQ ID NO. 29 or SEQ ID NO. 30;
the LCDR3 comprises one of the peptides whose amino acid sequences shown as SEQ ID NO. 31, SEQ ID NO. 32, SEQ ID NO. 33, SEQ ID NO. 34, SEQ ID NO. 35, SEQ ID NO. 36 or SEQ ID NO. 37. - View Dependent Claims (2, 5, 6, 7, 8, 9, 10, 11, 12)
- the LCDR1 comprises one of peptides whose amino acid sequences are shown as of SEQ ID NO. 18, SEQ ID NO. 19, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 23 or SEQ ID NO. 24;
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3. An anti-PD-1 monoclonal antibody comprises light chains and heavy chains, wherein the heavy chain contains three complementarity determining regions which are named as HCDR1, HCDR2 and HCDR3;
- the HCDR1 comprises one of peptides whose amino acid sequences were shown as SEQ ID NO. 38 or SEQ ID NO. 39;
the HCDR2 comprises one of peptides whose amino acid sequences were shown as SEQ ID NO. 40, SEQ ID NO. 41 or SEQ ID NO. 42;
the HCDR3 comprises one of peptides whose amino acid sequences were shown as SEQ ID NO. 43 or SEQ ID NO. 44. - View Dependent Claims (4)
- the HCDR1 comprises one of peptides whose amino acid sequences were shown as SEQ ID NO. 38 or SEQ ID NO. 39;
Specification