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METHOD FOR DETECTING MYCOPLASMA

  • US 20170240959A1
  • Filed: 09/07/2015
  • Published: 08/24/2017
  • Est. Priority Date: 09/10/2014
  • Status: Active Grant
First Claim
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1. A set of a forward primer, a reverse primer and a probe for detecting Mycoplasma in a test sample by a multiplex real time quantitative PCR, wherein the set contains one or more forward primers, two or more reverse primers and one or more probes;

  • the probe(s) is a probe for specifically detecting products amplified by use of the forward primer and the reverse primer;

    the forward primer(s) is an oligonucleotide consisting of a nucleotide sequence, which is selected from the group consisting of nucleotide sequences each consisting of continuous 17 to 30 nucleotides in the nucleotide sequence represented by SEQ ID No;

    1, and which contains a nucleotide sequence (caaggtatccc) at nucleotide positions 14 to 24 in SEQ ID No;

    1;

    the reverse primers each are an oligonucleotide consisting of a nucleotide sequence, which is selected from the group consisting of nucleotide sequences each consisting of continuous 17 to 26 nucleotides in one or more nucleotide sequences represented by SEQ ID Nos;

    14 and 17 to 20; and

    the probe(s) is an oligonucleotide, which consists of a nucleotide sequence selected from the group consisting of nucleotide sequences each consisting of continuous 17 to 26 nucleotides in the nucleotide sequence represented by SEQ ID No;

    33, or which consists of a complementary nucleotide sequence thereto.

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