METHOD FOR DETECTING MYCOPLASMA
First Claim
1. A set of a forward primer, a reverse primer and a probe for detecting Mycoplasma in a test sample by a multiplex real time quantitative PCR, wherein the set contains one or more forward primers, two or more reverse primers and one or more probes;
- the probe(s) is a probe for specifically detecting products amplified by use of the forward primer and the reverse primer;
the forward primer(s) is an oligonucleotide consisting of a nucleotide sequence, which is selected from the group consisting of nucleotide sequences each consisting of continuous 17 to 30 nucleotides in the nucleotide sequence represented by SEQ ID No;
1, and which contains a nucleotide sequence (caaggtatccc) at nucleotide positions 14 to 24 in SEQ ID No;
1;
the reverse primers each are an oligonucleotide consisting of a nucleotide sequence, which is selected from the group consisting of nucleotide sequences each consisting of continuous 17 to 26 nucleotides in one or more nucleotide sequences represented by SEQ ID Nos;
14 and 17 to 20; and
the probe(s) is an oligonucleotide, which consists of a nucleotide sequence selected from the group consisting of nucleotide sequences each consisting of continuous 17 to 26 nucleotides in the nucleotide sequence represented by SEQ ID No;
33, or which consists of a complementary nucleotide sequence thereto.
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Abstract
An object of the present invention is to provide a detection method for Mycoplasma by which a greater number of Mycoplasma species can be more quickly and easily detected with high sensitivity and accuracy, a set of a forward primer, a reverse primer and a probe for the detection and a kit containing the set. Mycoplasma in a test sample is detected by a multiplex real time quantitative PCR using one or more forward primers, each of which is an oligonucleotide consisting of a nucleotide sequence, which is selected from the group consisting of nucleotide sequences each consisting of continuous 17 to 30 nucleotides in the nucleotide sequence represented by SEQ ID No: 1, and which contains a nucleotide sequence at nucleotide positions 14 to 24 in SEQ ID No: 1; reverse primers, each of which is an oligonucleotide consisting of a nucleotide sequence, which is selected from the group consisting of nucleotide sequences each consisting of continuous 17 to 26 nucleotides in the one or more nucleotide sequences represented by SEQ ID Nos: 14 and 17 to 20; and a probe(s), which is an oligonucleotide, which consists of a nucleotide sequence selected from the group consisting of nucleotide sequences each consisting of continuous 17 to 26 nucleotides in the nucleotide sequence represented by SEQ ID No: 33, or which consists of a complementary nucleotide sequence thereto.
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20 Claims
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1. A set of a forward primer, a reverse primer and a probe for detecting Mycoplasma in a test sample by a multiplex real time quantitative PCR, wherein the set contains one or more forward primers, two or more reverse primers and one or more probes;
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the probe(s) is a probe for specifically detecting products amplified by use of the forward primer and the reverse primer; the forward primer(s) is an oligonucleotide consisting of a nucleotide sequence, which is selected from the group consisting of nucleotide sequences each consisting of continuous 17 to 30 nucleotides in the nucleotide sequence represented by SEQ ID No;
1, and which contains a nucleotide sequence (caaggtatccc) at nucleotide positions 14 to 24 in SEQ ID No;
1;
the reverse primers each are an oligonucleotide consisting of a nucleotide sequence, which is selected from the group consisting of nucleotide sequences each consisting of continuous 17 to 26 nucleotides in one or more nucleotide sequences represented by SEQ ID Nos;
14 and 17 to 20; and
the probe(s) is an oligonucleotide, which consists of a nucleotide sequence selected from the group consisting of nucleotide sequences each consisting of continuous 17 to 26 nucleotides in the nucleotide sequence represented by SEQ ID No;
33, or which consists of a complementary nucleotide sequence thereto. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification