ULTRASOUND TECHNOLOGY TO CONTROL THE SPATIAL ORGANIZATION OF CELLS AND PROTEINS IN ENGINEERED TISSUES

• US 20170260504A1
• Filed: 05/26/2017
• Published: 09/14/2017
• Est. Priority Date: 05/19/2009
• Status: Active Grant

• Current Assignee: University of Rochester
• Original Assignee: University of Rochester
• Inventors: DALECKI, Diane, HOCKING, Denise, GARVIN, Kelley
• Granted Patent: US 10,640,750 B2
• Time in Patent Office: Days
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 9/00 Medicinal preparations characterised by special physical form nuclear magnetic resonance contrast preparations or magnetic resonance imaging contrast preparations A61K49/18; preparations containing radioactive substances A61K51/12
• CPC Class Codes: C12M 35/04 Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
• CPC Class Codes: C12N 2521/10 Sound, e.g. ultrasounds
• CPC Class Codes: C12N 2533/52 Fibronectin; Laminin
• CPC Class Codes: C12N 2533/54 Collagen; Gelatin
• CPC Class Codes: C12N 5/0062 General methods for three-dimensional culture
• CPC Class Codes: C12N 5/0656 Adult fibroblasts
• CPC Class Codes: C12N 5/069 Vascular Endothelial cells
• CPC Class Codes: C12Q 1/02 involving viable microorganisms
• CPC Class Codes: C12Q 1/68 involving nucleic acids

Abstract

The present invention is directed to methods of inducing spatial organization of cells an in vitro culture system using ultrasound technology. The invention is further directed to methods of inducing extracellular matrix remodeling and neovessel formation in an in vitro culture system and generating vascularized engineered tissue constructs using ultrasound technology.

Claims

1. A vascularized engineered tissue construct, wherein said vascularized engineered tissue construct has a thickness in three-dimensions of at least 2 mm.

2. The vascularized engineered tissue construct of claim 1, wherein the vascularized engineered tissue construct is selected from the group consisting of a muscular construct, a vascular construct, an esophageal construct, an intestinal construct, a rectal construct, an ureteral construct, a cartilaginous construct, a cardiac construct, a liver construct, a bladder construct, a kidney construct, a pancreatic construct, a skeletal construct, a filamentous/ligament construct, a lung construct, a neural construct, a bone construct, and a skin construct.

3. A method of screening a test agent, said method comprising:

providing a test agent;

providing the vascularized engineered tissue construct of claim 1;

contacting the test agent with the vascularized engineered tissue construct; and

assaying one or more biological endpoints in the vascularized engineered tissue construct.

4. The method according to claim 3, wherein the one or more biological endpoints are selected from the group consisting of carcinogenicity, cell death, cell proliferation, gene expression, protein expression, cellular metabolism, and any combination thereof.

5. A method of treating a subject in need of a tissue repair or tissue replacement, said method comprising:

selecting a subject in need of tissue repair or tissue replacement;

providing the vascularized tissue engineered construct of claim 1; and

implanting the vascularized engineered tissue construct into the selected subject.