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Probe Density Considerations and Elongation of Self-Complementary Looped Probes Where Probes Are Attached to a Solid Phase

  • US 20170268050A1
  • Filed: 05/31/2017
  • Published: 09/21/2017
  • Est. Priority Date: 04/12/2006
  • Status: Abandoned Application
First Claim
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1. A method of enhancing the sensitivity of detection of a nucleic acid target, in solution, by formation of a duplex between a nucleic acid target and the target binding domain of an oligonucleotide probe, said probe having a target binding domain and a complementary closing domain capable of forming a duplex with the target binding domain, and a joining region between the target binding domain and the closing domain, the method comprising:

  • attaching the probe to the outer surface of a bead at a lateral density on the surface exceeding a predetermined minimum, wherein the predetermined minimum is determined such that the distance between the occupied probe site and the nearest unoccupied site permits target release and re-capture on a time-scale less than time for target escape into solution.

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