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PREPARATION OF ADAPTER-LIGATED AMPLICONS

  • US 20170283869A1
  • Filed: 08/21/2015
  • Published: 10/05/2017
  • Est. Priority Date: 09/05/2014
  • Status: Active Grant
First Claim
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1. A method of preparing adapter-ligated amplicons, comprising the steps of:

  • (i) contacting double-stranded amplicons withat least one polynucleotide kinase,at least one DNA ligase, andDNA adapter molecules,to obtain a reaction mixture,(ii) incubating said reaction mixture under conditions simultaneously allowinga 5′

    phosphorylation of said double-stranded amplicons by said polynucleotide kinase, anda joining of said DNA adapter molecules to at least one end of said double-stranded amplicons by said DNA ligase,to obtain adapter-ligated amplicons.

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