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METHODS FOR DETECTION OF NUCLEOTIDE MODIFICATION

  • US 20170283870A1
  • Filed: 09/07/2015
  • Published: 10/05/2017
  • Est. Priority Date: 09/05/2014
  • Status: Abandoned Application
First Claim
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1. A method of identifying a 5-formylcytosine residue in a sample nucleotide sequence comprising;

  • (i) providing a population of single stranded polynucleotides which comprise the sample nucleotide sequence,(ii) reducing a first portion of said population by adding an alkaline borohydride solution,(iii) treating the reduced first portion of said population and a second portion of said population with bisulfite,(iv) sequencing the polynucleotides in the first and second portions of the population following steps ii) and iii) to produce first and second nucleotide sequences, respectively and;

    (v) identifying the residues in the first and second nucleotide sequences which correspond to a 5-formylcytosine residue in the sample nucleotide sequence.

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