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METHOD AND APPARATUS FOR PERFORMING CONTACTLESS OPTICALLY-INDUCED DIELECTROPHORESIS FOR SEPARATION OF CIRCULATING TUMOR CELLS

  • US 20170297036A1
  • Filed: 12/01/2016
  • Published: 10/19/2017
  • Est. Priority Date: 04/19/2016
  • Status: Active Grant
First Claim
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1. A method for performing contactless optically-induced dielectrophoresis (ODEP) for separation of circulating tumor cells (CTCs), the method comprising the steps of:

  • (1) obtaining blood with rare cell suspected CTCs.;

    (2) adding at least one fluorescent antibody binding to CTCs into the blood;

    (3) staining the blood;

    (4) injecting the stained blood with fluorescent dye into an ODEP device and then performing fluorescent image identification.;

    (5) trapping the CTCs with at least one fluorescent antibody in the ODEP device by creating an image pattern and then generating an ODEP force.;

    (6) Separating the trapped CTCs from other non-CTCs cells;

    (7) absorbing the trapped CTCs; and

    (8) obtaining a high purity of CTCs after repeating steps (5) and (6).

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