METHOD AND APPARATUS FOR PERFORMING CONTACTLESS OPTICALLY-INDUCED DIELECTROPHORESIS FOR SEPARATION OF CIRCULATING TUMOR CELLS
First Claim
1. A method for performing contactless optically-induced dielectrophoresis (ODEP) for separation of circulating tumor cells (CTCs), the method comprising the steps of:
- (1) obtaining blood with rare cell suspected CTCs.;
(2) adding at least one fluorescent antibody binding to CTCs into the blood;
(3) staining the blood;
(4) injecting the stained blood with fluorescent dye into an ODEP device and then performing fluorescent image identification.;
(5) trapping the CTCs with at least one fluorescent antibody in the ODEP device by creating an image pattern and then generating an ODEP force.;
(6) Separating the trapped CTCs from other non-CTCs cells;
(7) absorbing the trapped CTCs; and
(8) obtaining a high purity of CTCs after repeating steps (5) and (6).
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Accused Products
Abstract
A method for performing contactless ODEP for separation of CTCs is provided with the steps of obtaining patients'"'"' blood with rare cell suspected CTCs; adding at least one fluorescent antibody binding to CTCs into the blood; staining the blood; injecting the stained blood with fluorescent dye into an ODEP device and then performing fluorescent image identification; trapping the CTCs with at least one fluorescent antibody in the ODEP device by creating an image pattern and then generating an ODEP force; Separating the trapped CTCs from other non-CTCs cells; absorbing the trapped CTCs; and obtaining a high purity of CTCs. An apparatus for performing contactless ODEP for separation of CTCs is also provided.
3 Citations
13 Claims
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1. A method for performing contactless optically-induced dielectrophoresis (ODEP) for separation of circulating tumor cells (CTCs), the method comprising the steps of:
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(1) obtaining blood with rare cell suspected CTCs.; (2) adding at least one fluorescent antibody binding to CTCs into the blood; (3) staining the blood; (4) injecting the stained blood with fluorescent dye into an ODEP device and then performing fluorescent image identification.; (5) trapping the CTCs with at least one fluorescent antibody in the ODEP device by creating an image pattern and then generating an ODEP force.; (6) Separating the trapped CTCs from other non-CTCs cells; (7) absorbing the trapped CTCs; and (8) obtaining a high purity of CTCs after repeating steps (5) and (6). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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Specification