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METHODS OF DIFFERENTIATING STEM CELLS INTO LIVER CELL LINEAGES

  • US 20170304369A1
  • Filed: 10/08/2015
  • Published: 10/26/2017
  • Est. Priority Date: 10/08/2014
  • Status: Abandoned Application
First Claim
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1. A method of differentiating cells of the definitive endoderm (DE) lineage into posterior foregut lineage comprising contacting said stem cells with:

  • one or more retinoic acid activators; and

    /or one or more inhibitors of TGFβ

    signaling, optionally further comprising contacting said stem cells with one or more activators of BMP signaling, optionally further comprising contacting said stem cells with one or more activators of FGF signaling, optionally wherein the cells of the posterior foregut lineage comprise elevated gene expression of posterior foregut lineage markers and decreased expression of dorsal foregut markers relative to undifferentiated cells, optionally wherein the duration of the method is about 1 to 84 hours.

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